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Cloned

T4 Polynucleotide Kinase

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2021A
T4 Polynucleotide Kinase
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U
71,200¿ø 
89,000¿ø
°¡°ÝÇÒÀÎ
03.02 ~ 04.30
2021A_DS.v1901Da.pdf
Takara
2021B
T4 Polynucleotide Kinase
°ü·ÃÇмú ±¸¸ÅÇϱâ
5,000 U
400,500¿ø 

³óµµ 10 U/§¡
ÇüÅÂ
50 mM Tris-HCl (pH7.5)
50 mM KCl
1 mM DTT
0.1 §­ ATP
50% Glycerol
º¸Á¸ -20¡É
÷ºÎ Buffer Á¶¼º(10¡¿)
500 mM Tris-HCl (pH8.0)
100 mM MgCl2
50 mM DTT
Ç¥½ÄÇÏÁö ¾Ê°í ÀλêÈ­ ¹ÝÀÀÀ» ½Ç½ÃÇÒ ¶§´Â ¹ÝÀÀ¾×¿¡ ÃÖÁ¾³óµµ 1 mMÀÌ µÇµµ·Ï ATP¸¦ ÷°¡ÇØ ÁֽʽÿÀ.
´Ü, º» È¿¼Ò¿¡ ATP´Â ÷ºÎµÇÁö ¾Ê½À´Ï´Ù.
±â¿ø
Escherichia coli carrying the high expression system of T4 pseT gene
Á¦Ç°¼³¸í
º» È¿¼Ò´Â DNA ¶Ç´Â RNAÀÇ 5¡¯ ¸»´Ü¿¡ ATPÀÇ ¥ãÀ§Ä¡¿¡ Àλê±â¸¦ ÷°¡ÇÏ´Â È¿¼ÒÀÌ´Ù. ATP Á¸ÀçÇÏ¿¡¼­´Â 5¡¯- OH ¸»´ÜÀÇ ÀλêÈ­ ¹ÝÀÀ(ÀλêÈ­ ÀüÀ̹ÝÀÀ)ÀÌ ÀϾÁö¸¸, 5¡¯-P ¸»´ÜÀÌ ±âÁúÀÎ °æ¿ì ADP°¡ Á¸ÀçÇÏ¸é ¿ª¹ÝÀÀÀÌ ÀϾ 5¡¯ ¸»´Ü¿¡ Å»ÀλêÈ­ ¹ÝÀÀÀÌ ÀϾ´Ù. À̶§, Ç¥½ÄÇÑ ATP¸¦ ÷°¡Çϸé ÀλêÀüÀ̹ÝÀÀµµ µ¿½Ã¿¡ ÀϾ 5¡¯ ¸»´ÜÀÌ Ç¥½ÄµÈ °Í°ú ±³È¯ÀÌ ÀϾ´Ù(±³È¯¹ÝÀÀ).
Ȱ¼ºÀÇ Á¤ÀÇ
ÇüÁúÀüȯ È¿À²
Micrococcal nuclease·Î Ȱ¼ºÈ­ ÇÑ ¼Û¾ÆÁö Èä¼± DNA¸¦ ±âÁú·Î ÇÏ¿© 37¡É, pH7.6¿¡¼­ 30ºÐ µ¿¾È 1 nmolÀÇ¡² ¥ã-32P¡³ATP¸¦ »êºÒ¿ë¼º ħÀü¹°·Î ¹Ù²Ù´Â È¿¼ÒȰ¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
Ȱ¼ºÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º
50 mM Tris-HCl (pH7.6)
10 mM MgCl2
10 mM 2-Mercaptoethanol
100 ¥ìM ¡²¥ã-32P¡³ATP
0.2 mg/ml 5¡¯-OH DNA
¼øµµ
- 10 UÀÇ º» È¿¼Ò¿Í 1 §¶ÀÇ ¥ëDNA-Hind III ºÐÇØ¹°À» 37¡É, 24½Ã°£ ¹ÝÀÀÇÏ¿©µµ DNAÀÇ Àü±â¿µµ¿ ÆÐÅÏ¿¡ º¯È­°¡ ¾ø´Ù.
- 1 UÀÇ º» È¿¼Ò¿Í 1 §¶ÀÇ 16S¿Í 23S rRNA¸¦ 37¡É, 24½Ã°£ ¹ÝÀÀÇÏ¿©µµ RNAÀÇ Àü±â¿µµ¿ ÆÐÅÏ¿¡ º¯È­°¡ ¾ø´Ù.
¿ëµµ
- DNA¿Í RNAÀÇ 5¡¯¸»´ÜÀÇ Ç¥½Ä
- ÇÕ¼º DNA linkerÀÇ 5¡¯¸»´ÜÀÇ ÀλêÈ­
°ü·ÃÁ¦Ç°
MEGALABEL (TaKaRa Code 6070)

Keyword :

¼ö½ÄÈ¿¼Ò£¯Alkaline Phosphatase¡¢Polynucleotide Kinase