¿À´Ã ÇÏ·ç ±×¸¸º¸±â
´Ý±â
·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø¼¾ÅÍ
¾÷¹«¾È³»¦¢ÇмúÁö¿ø
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæÃ»Áö¿ª ÁÖ¹®, ÇмúÁö¿ø
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Products
°øÁö»çÇ×
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
樨毢
ȸ»ç¼Ò°³
That's Good Science!
Á¦Ç°¼±Åð¡À̵å
ÀüÁ¦Ç°º¸±â
Custom Service
COVID-19
À̺¥Æ®/Ä·ÆäÀÎ
´ÙÄ«¶ó¼Ò½Ä
´ÙÄ«¶ó°ø½Ã
½ÅÁ¦Ç°Á¤º¸
´ÙÄ«¶ó ¶óÀ̼±½º
±â¼úÁö¿ø¹®ÀÇ
Á¤º¸ÀÚ·á½Åû
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LS&B
ºê·Î¼Å
eNews
CoA & MSDS
¿Â¶óÀÎÅø
樨毢
¿Â¶óÀμ¼¹Ì³ª
ȸ»ç¼Ò°³
´ë¸®Á¡¾È³»
ã¾Æ¿À½Ã´Â±æ
Welcome
About Thats Good Science
Watch the Videos - S3
Watch the Videos - S2
Watch the Videos - S1
Home
>
ÀüÁ¦Ç°º¸±â
>
Cloning °ü·Ã
>
¼ö½ÄÈ¿¼Ò£¯Ligase
> T4 RNA Ligase
T4 RNA Ligase
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Takara
2050A
T4 RNA Ligase
1,000 U
136,800¿ø
171,000¿ø
03.02 ~ 04.30
Takara
2050B
T4 RNA Ligase
5,000 U
769,500¿ø
Product index
½Ä¹°¿ë Vectors
Takara Plant ½ÇÇè °ü·Ã..
Agrobacterium tumefaci..
pRI 101 DNA ½Ã¸®Áî
pRI 201 DNA ½Ã¸®Áî
pRI 909/910 DNA
È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
pAUR101 DNA
pAUR112 DNA
pAUR123 DNA
pAUR135 DNA
pAUR224 DNA
pAUR316 DNA
pPTR I DNA
pPTR II DNA
Genomic DNA
Genomic DNA
Thermus thermophilus H..
¥ëDNA
¥õX174 RF I DNA
DNA Ligation
Cloning ½ÇÇè ÀÔ¹®ÀÚ¸¦ ..
Takara Cloning FAQ
½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Blunting Kit
DNA Ligation Kit
DNA Ligation Kit £¼Mig..
TaKaRa DNA Ligation Ki..
TA-Cloning, ÆòȰ¸»´Ü Cloni..
½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Fragmentation Kit
Kilo-Sequence ¿ë Delet..
Mighty Cloning Reagent..
Mighty TA-cloning Kit
Mighty TA-cloning Reag..
TaKaRa LA PCR¢â in vit..
TaKaRa T-Vector pMD20
In-Fusion Cloning
[Overview] In-Fusion¢ç..
[Àû¿ë] In-Fusion¢ç HD ..
Online Tools for In-Fu..
[FAQ] In-Fusion¢ç HD C..
½ÇÁ¦ °í°´ÀÇ »ç¿ëÈıâ
2ź ½ÇÁ¦ »ç¿ëÀÚ Èıâ
[°³¿ä] So Easy Product
[NEW] In-Fusion¢ç Snap..
[Kit] In-Fusion¢ç Clon..
[Kit] In-Fusion¢ç EcoD..
[Plus] In-Fusion¢ç Clo..
[Plus] In-Fusion¢ç Eco..
In-Fusion¢ç Ready Vect..
Genomic DNA ÁõÆø¿ë Kit
GenomeWalkerKit
Vector (Cloning ¿ë)
M13 mp18 Single Strand..
pBR322 DNA
pHY300PLK DNA
pKF18k-2/19k-3 DNA
pSTV28/29 DNA
pTWV228 DNA
pUC118/119 type vector
pUC18/19 type vector
TaKaRa T-Vector pMD20
Competent Cells & Electro-..
Takara Competent Cell ..
Agrobacterium tumefaci..
Chaperone Competent Ce..
E. coli Competent Cell..
E. coli Competent Cell..
E. coli Electro-Cells ..
¼ö½ÄÈ¿¼Ò¿¡ ´ëÇÏ¿©
¼ö½ÄÈ¿¼Ò ¼øµµ À϶÷
¼ö½ÄÈ¿¼Òº° ¿ëµµ
¼ö½ÄÈ¿¼Ò£¯Ligase
E. coli DNA Ligase
T4 DNA Ligase
T4 RNA Ligase
¼ö½ÄÈ¿¼Ò£¯Alkaline Phospha..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
T4 Polynucleotide Kina..
¼ö½ÄÈ¿¼Ò£¯DNA Polymerase¡¢..
DNA Polymerase I (E. ..
Klenow Fragment (Large..
Poly(A) Polymerase
SP6 RNA Polymerase
T4 DNA Polymerase
T7 RNA Polymerase
Terminal Deoxynucleoti..
¼ö½ÄÈ¿¼Ò£¯Nuclease¡¢Topois..
BAL 31 Nuclease
DNA Topoisomerase I
Exonuclease I
Exonuclease III
Micrococcal Nuclease
Mung Bean Nuclease
Recombinant DNase I (R..
Ribonuclease H (RNase ..
S1 Nuclease
Sequencing Primer
M13 Primers
Nucleotide
Deoxyribonucleoside 8..
dNTP Mixture
Ribonucleoside 5£§-Tri..
¹èÁö ¡¤ Buffer (ºÐ¸»)
AccuGENE 20¡¿SSPE Buff..
Bovine Serum Albumin S..
Dulbecco£§s Formula£¨M..
IPTG£¨dioxane free£©£¨..
TBE (Tris-borate-EDTA)..
Tris-Glycine-SDS powde..
X-Gal£¨5-Bromo-4-Chlor..
±× ¿Ü (À¯Àü°øÇÐ °ü·Ã)
DNA-OFF¢â
Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase (°è»ó±Õ ¼¼Æ÷..
³óµµ 10~50 U/§¡
Çü»ó
20 mM
Tris-HCl (pH7.5)
50 mM
NaCl
5 mM
2-Mercaptoethanol
0.1 mM
EDTA
50%
Glycerol
º¸Á¸ -20¡É
÷ºÎ Buffer Á¶¼º(10¡¿)
500 mM
Tris-HCl (pH7.5)
100 mM
MgCl
2
100 mM
DTT
10 mM
ATP
0.1%
BSA [º°Ã·]*
* BSA°¡ ħÀüÇÒ ¿ì·Á°¡ ÀÖÀ¸¹Ç·Î µ¿°á¡¤À¶ÇØ´Â °¡´ÉÇÑ ÇÑ ÇÇÇØ ÁֽʽÿÀ.
±â¿ø
Escherichia coli B infected with T4 am N82
Á¦Ç°¼³¸í
º» È¿¼Ò´Â ATP¸¦ AMP¿Í PPi·Î ºÐÇØÇÏ¿© ±× ¿¡³ÊÁö·Î 5¡¯-P¸»´ÜÀÇ oligonucleotide¿Í 3¡¯-OH ¸»´ÜÀÇ oligonuceotide¸¦ °áÇÕ½ÃŰ´Â È¿¼ÒÀÌ´Ù.
ÃÖ¼Ò±âÁúÀº NpNpNOH(3¡¯-OH oligomer, ¼ö¿ëü), pNp(5¡¯, 3¡¯DP monomer °ø¿©Ã¼)ÀÌ´Ù. ¶óÀ̰ÔÀÌ¼Ç È¿À²Àº ¼ö¿ëü, °ø¿©Ã¼ °¢°¢ÀÇ ¿°±â¿¡ ¿µÇâÀ» ¹ÞÀ¸¸ç, ÀϹÝÀûÀ¸·Î ¼ö¿ëü¿¡´Â purineÀ», °ø¿©Ã¼´Â pyrimidineÀ» Æ÷ÇÔÇÏ´Â °ÍÀÌ È¿À²ÀÌ ÁÁ´Ù. DNA´Â RNAº¸´Ù »ó´çÈ÷ È¿À²ÀÌ ¶³¾îÁö°í, ±¸Á¶ÀûÀ¸·Î 3¡¯¸»´Ü¿¡ °¡±î¿î P ¸»´ÜÀ» °®´Â RNAµµ È¿À²ÀÌ ÁÁÁö ¾ÊÀ¸¸ç, 3¡¯- uridine oligomer ¶ÇÇÑ ¼ö¿ëü·Î½á ºÎÀû´çÇÏ´Ù. 3¡¯¼ö¿ëüÀÇ °æ¿ì´Â BAP󸮿¡ ÀÇÇÏ¿© È¿À²À» ³ôÀÏ ¼ö ÀÖ´Ù.
5¡¯, 3¡¯DP monomer °ø¿©Ã¼·Î½á´Â C £¾ U £¾ A £¾ GÀÇ ¼øÀ¸·Î ³ôÀº È¿À²À» °¡Áö°í ÀÖ´Ù.
Ȱ¼ºÀÇ Á¤ÀÇ
Oligo(A)nÀ» ±âÁú·Î ÇÏ¿© 5¡É, 10ºÐµ¿¾È 1 pmolÀÇ [5¡¯-
32
P]pCp¸¦ »êºÒ¿ë¼º ħÀü¹°·Î »ç¿ëÇÏ´Â °ÍÀ» È¿¼ÒȰ¼º 1 U·Î ÇÑ´Ù(RNA 3¡¯¸»´Ü Ç¥½Ä¹ÝÀÀ).
Ȱ¼ºÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º
50 mM
HEPES-NaOH Buffer (pH7.5)
20 mM
MgCl
2
3.3 mM
DTT
6 ¥ìM
ATP
0.001%
BSA
10%
DMSO
1.2 ¥ìM
3¡¯- OH RNA
2.4 ¥ìM
[5¡¯-
32
P]pCp
¼øµµ
- 100 UÀÇ º» È¿¼Ò¿Í 1 §¶ÀÇ ¥ëDNA-Hind III ºÐÇØ¹°À» 37¡É, 24½Ã°£ ¹ÝÀÀÇØµµ DNAÀÇ Àü±â¿µµ¿ ÆÐÅÏ¿¡ º¯È°¡ ¾ø´Ù.
- 100 UÀÇ º» È¿¼Ò¿Í 1 §¶ÀÇ 16S¿Í 23S rRNA¸¦ 37¡É, 24½Ã°£ ¹ÝÀÀÇÏ¿©µµ RNAÀÇ Àü±â¿µµ¿ ÆÐÅÏ¿¡ º¯È°¡ ¾ø´Ù.
- 20 UÀÇ º» È¿¼Ò¿Í 6 §¶ÀÇ ¥ëDNA-Hind III ºÐÇØ¹° ¶Ç´Â 6 §¶ÀÇ ¥ëDNA-Pst I ºÐÇØ¹°À» 37¡É, 24½Ã°£ ¹ÝÀÀÇÏ¿©µµ °¢ DNA´Â T4 DNA ligase¿¡ ÀÇÇØ 90%ÀÌ»ó ¿¬°áµÇ°í, ±×Áß 100%°¡ °¢°¢ Hind III ¶Ç´Â Pst I¿¡ ÀÇÇØ ÀçÀý´ÜµÈ´Ù.
»ç¿ë»óÀÇ ÁÖÀÇ
- 4¡É ÀÌÇÏ º¸Á¸¿¡¼ ¾à 1³â°£ ¾ÈÁ¤ÇÏ´Ù.
- ºÐÀÚ°£ ¶óÀ̰ÔÀ̼ÇÀÇ ÃÖÀû¹ÝÀÀ ¿Âµµ´Â 5~15¡É·Î, ±× ÀÌ»ó ³ô¾ÆÁö¸é ¾ïÁ¦µÈ´Ù.
- PEG °øÁ¸ÇÏ¿¡¼ ¹ÝÀÀÀº ÃËÁøµÇ³ª, ¹ÝÀÀƯÀ̼º¿¡´Â º¯È°¡ ¾ø´Ù.
- ¿¬°á¹ÝÀÀÀÇ È¿À²À» ³ôÀ̱â À§Çؼ´Â È¿¼Ò³óµµ´Â ³ô°í ATP ³óµµ´Â ±âÁú³óµµ¸¦ °í·ÁÇÏ¿© °¡´ÉÇÑ ³·°Ô ÇÏ´Â °ÍÀÌ ÁÁ´Ù.
- 10¡¿ ÷ºÎ ¹öÆÛ¿¡ 0.1% BSA¸¦ Á÷Á¢ ÷°¡ÇÏ¸é ´Ù·®ÀÇ ¹é»öħÀüÀÌ »ý±â¹Ç·Î ¹ÝÀÀ¾×À» Á¶Á¦ÇÒ ¶§´Â ´ÙÀ½ÀÇ ¼ø¼·Î ½Ã¾àÀ» ÷°¡ÇÑ´Ù.
dH
2
O ¡æ 10¡¿ ÷ºÎ ¹öÆÛ ¡æ 0.1% BSA ¡æ ±âÁú RNA ¶Ç´Â DNA
¿ëµµ
- ´ÜÀϰ¡´Ú RNA¿Í ´ÜÀϰ¡´Ú DNAÀÇ 3¡¯-OH ¸»´ÜÀÇ Ç¥½Ä
- ´ÜÀϰ¡´Ú oligo RNA, ´ÜÀϰ¡´Ú DNAÀÇ ÇÕ¼º
- ¿ÏÀüÇÑ ±æÀÌÀÇ cDNA ÇÕ¼º
Âü°í
Yeast tRNA
phe
¸¦ ±âÁú·Î ÇÏ¿´À» ¶§, »ó±â ¹ÝÀÀ¾× Áß¿¡ 50 UÀÇ º» È¿¼Ò¸¦ 5¡É, 16½Ã°£ ó¸®Çϸé 80% ÀÌ»ó tRNApheÀÇ 3¡¯¸»´ÜÀÌ Ç¥½ÄµÈ´Ù.
Keyword :
¼ö½ÄÈ¿¼Ò£¯Ligase
Cloned
E. coli DNA Ligase
Cloning¿ë ligase
T4 DNA Ligase
T4 RNA Ligase