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Gene transfer (virus)
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Retrovirus
> pDON-AI-2 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vector
pDON-AI-2 DNA
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Á¦Ç°ÄÚµå
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(ºÎ°¡¼¼º°µµ)
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Takara
3653
pDON-AI-2 Neo DNA
20 ¥ìg
511,200¿ø
639,000¿ø
09.02 ~ 10.31
Takara
3654
pDON-AI-2 DNA
20 ¥ìg
511,200¿ø
639,000¿ø
09.02 ~ 10.31
Product index
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Á¦Ç°¼³¸í
pDON-AI-2 DNA´Â °íÈ¿À² À¯ÀüÀÚ µµÀÔ¿ë retrovirus vectorÀÌ´Ù. º» vector´Â LTR°ú packaging signal (¥× ¼¿) ÀÌ¿ÜÀÇ MoMLV À¯·¡ À¯ÀüÀÚ (gag, pol, env)¸¦ Æ÷ÇÔÇÏÁö ¾Ê°í 5¡¯LTRÀÇ U3 ¿µ¿ªÀÌ º¸´Ù °·ÂÇÑ cytomegalovirus À¯·¡ÀÇ promoter·Î ġȯµÇ¾î Àֱ⠶§¹®¿¡ Àü»ç È¿À²ÀÌ ³ô´Ù. µû¶ó¼ °í¿ª°¡ÀÇ ÀçÁ¶ÇÕ retrovirus¸¦ ¾òÀ» ¼ö ÀÖ´Ù. ¶ÇÇÑ ¼¼Æ÷¿¡ µµÀÔÇÑ ÈÄÀÇ ¸ñÀûÀ¯ÀüÀÚÀÇ ¹ßÇö È¿À²À» ³ôÀ̱â À§ÇÏ¿© cloning ºÎÀ§ ¾ÕÂÊ¿¡ human actin À¯·¡ÀÇ intron °ú splice acceptor°¡ À§Ä¡ÇÏ°í ÀÖ´Ù. ¶ÇÇÑ, pDON-AI-2 Neo DNA (Code 3653)´Â selection marker·Î½á neomycin ³»¼ºÀ¯ÀüÀÚ¸¦ °¡Áö°í ÀÖ´Ù.
º» Á¦Ç°À» ÀÌ¿ëÇϸé pMEI-5 ½Ã¸®Áî¿¡ ºñÇØ °í¿ª°¡ÀÇ ÀçÁ¶ÇÕ retrovirus¸¦ ¾òÀ» ¼ö ÀÖ°í, È¿À²ÀûÀÎ À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÏ´Ù.
(º» vector´Â S. Kim¿¡ ÀÇÇØ °³¹ßµÈ retrovirus vector Á¦ÀÛ¿ë plasmid vector pDON-AI DNA¸¦ º¯ÇüÇÑ °ÍÀÌ´Ù.)
Retrovirus¸¦ ÀÌ¿ëÇÑ À¯ÀüÀÚµµÀÔÀÇ Æ¯Â¡
Ç¥Àû¼¼Æ÷¿¡ ¸ñÀûÀ¯ÀüÀÚ¸¦ µµÀÔÇÏ¿© ¹ßÇö½ÃÅ°´Â ¹æ¹ý¿¡´Â electroporation µîÀÇ ¹°¸®Àû ¹æ¹ý°ú DEAE-dextranÀ̳ª ¸®Æ÷Á» (liposome), calciumphosphate µîÀ» ÀÌ¿ëÇÏ´Â ÈÇÐÀû ¹æ¹ýÀÌ ÀÖ´Ù. ÀÌ ¿Ü¿¡µµ ¹ÙÀÌ·¯½º º¤Å͸¦ ÀÌ¿ëÇÑ ¿Ü·¡ À¯ÀüÀÚÀÇ °íÈ¿À² µµÀÔ¹ý ¿¬±¸µµ ÁøÇàµÇ°í ÀÖÀ¸¸ç, ÇöÀç »ç¿ëµÇ°í ÀÖ´Â ´ëÇ¥ÀûÀÎ ¹ÙÀÌ·¯½º º¤ÅÍ¿¡´Â ·¹Æ®·Î¹ÙÀÌ·¯½º, ¾Æµ¥³ë¹ÙÀÌ·¯½º(adenovirus), adenovirus associated virus, vaccinia virus µîÀÌ ÀÖ´Ù. ƯÈ÷ ·¹Æ®·Î¹ÙÀÌ·¯½º º¤Å͸¦ ÀÌ¿ëÇÑ À¯ÀüÀÚ µµÀÔ¿¡´Â ´ÙÀ½°ú °°Àº ÀåÁ¡ÀÌ ÀÖ´Ù.
µµÀÔÀ¯ÀüÀÚ°¡ ¿°»öü¿¡ ÀçÁ¶ÇյǹǷΠÀå±â°£ ¾ÈÁ¤ÀûÀ¸·Î À¯ÀüÀÚ¸¦ ¹ßÇö½Ãų ¼ö ÀÖ´Ù. ¹Ý¸é, µµÀÔÀ¯ÀüÀÚ°¡ ¿°»öü¿¡ »ðÀÔµÇÁö ¸øÇÑ °æ¿ì ¼¼Æ÷ ³»¿¡¼ ºÐÇصǰųª ¼¼Æ÷ºÐ¿°úÁ¤¿¡¼ Èñ¼®µÇ¾î ¹ö¸®¹Ç·Î À¯ÀüÀÚ ¹ßÇöÀÌ ÀÏ°ü¼º ÀÖ°Ô Áö¼ÓµÉ ¼ö ¾ø´Ù. ·¹Æ®·Î¹ÙÀÌ·¯½º º¤ÅÍ´Â ¿°»öü¿¡ ÀçÁ¶ÇյǹǷΠºÐ¿ ÈÄ¿¡µµ ¾ÈÁ¤ÀûÀ¸·Î Àü´ÞµÈ´Ù.
·¹Æ®·Î¹ÙÀÌ·¯½º ÀÌ¿ÜÀÇ ´Ù¸¥ ¹ÙÀÌ·¯½º º¤ÅÍ´Â Á¦ÀÛ°úÁ¤ÀÌ ¸Å¿ì º¹ÇÕÇÏÁö¸¸ ·¹Æ®·Î¹ÙÀÌ·¯½º´Â ºñ±³Àû ½±°Ô ¹ÙÀÌ·¯½º º¤Å͸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.
Áõ½Ä±â¿¡ ÀÖ´Â ¸¹Àº ¼¼Æ÷Á¾¿¡ À¯ÀüÀÚ¸¦ µµÀÔÇÒ ¼ö ÀÖ´Ù. ƯÈ÷, ¹°¸® ¡¤ ÈÇÐÀû ¹æ¹ýÀ¸·Î´Â À¯ÀüÀÚ µµÀÔÀÌ ¾î·Á¿ü´ø Á¶Ç÷¸ð¼¼Æ÷¿¡ RetroNectin(Fibronectin Fragment) (TaKaRa Code T100A/B)À» ÀÌ¿ëÇÔÀ¸·Î½á ·¹Æ®·Î¹ÙÀÌ·¯½º º¤Å͸¦ ¸Å°³·Î Á¶Ç÷¸ð¼¼Æ÷¿¡ À¯ÀüÀÚ¸¦ °íÈ¿À²·Î µµÀÔÇÒ ¼ö ÀÖ°Ô µÇ¾ú´Ù.
Çü»ó
10mM Tris-HCl (pH8.0)
1mM EDTA
±æÀÌ
pDON-AI-2 Neo DNA : 5,719 bp
pDON-AI-2 DNA : 4,586 bp
pDON-AI-2 DNAÀÇ ±¸Á¶
»ç¿ë»óÀÇ ÁÖÀÇ
pDON-5-DNA, pMEI-5 DNA¸¦ »ç¿ë½Ã ´ÙÀ½ »çÇ׿¡ ÁÖÀÇÇÏ¿© ÁֽʽÿÀ.
1. º» Á¦Ç°Àº ¿¬±¸ ¸ñÀû ÀÌ¿Ü¿¡´Â »ç¿ëÇÒ ¼ö ¾ø½À´Ï´Ù. Àΰ£, µ¿¹°ÀÇ ÀÇ·á, ÀÓ»ó Áø´Ü µî¿¡´Â »ç¿ëÇÏÁö ¾Êµµ·Ï ÁÖÀÇÇÏ¿© ÁֽʽÿÀ.
(º» Á¦Ç°¿¡ ÀÇÇØ ¾ò¾îÁø »ý¹°Àç·á¸¦ Á¦3ÀÚ¿¡°Ô ¾çµµÇÒ ¼ö ¾ø½À´Ï´Ù.) ¶Ç, ½ÄÇ°, ÈÀåÇ°, »ýÈ°¿ëÇ° µîÀ¸·Î »ç¿ëÇÏÁö ¸¶½Ê½Ã¿À.
2. º» Á¦Ç°À» ¿¬±¸ ¸ñÀû ÀÌ¿Ü¿¡ »ç¿ëÇÏ´Â °æ¿ì¿¡´Â »çÀü¿¡ ´ç»ç¿¡ ¹®ÀÇÇϽʽÿÀ. 3. º» retrovirus vector¿¡ ÀÇÇØ »ý»êµÇ´Â ¹ÙÀÌ·¯½º »óû »ðÀÔ Á¶°¢¿¡´Â À§ÇèÇÑ ¹ÙÀÌ·¯½º°¡ Æ÷Ç﵃ À§ÇèÀÌ Àֱ⠶§¹®¿¡ ÀçÁ¶ÇÕ retrovirusÀÇ »ý»ê°ú
Ãë±Þ¿¡´Â ÀûÀýÇÑ Á¶Ä¡¸¦ ÃëÇÒ ÇÊ¿ä°¡ ÀÖ½À´Ï´Ù. ÀÌ¿ë ½Ã °üÇÒ ºÎó ¹× Á¶Á÷ ¾ÈÀüÀ§¿øȸ°¡ Á¤ÇÑ ÀçÁ¶ÇÕ DNA½ÇÇè ÁöħÀ» µû¶ó ÁֽʽÿÀ.
4. º» Á¦Ç°ÀÇ »ç¿ëÀ¸·Î ÀÎÇÑ ¾î¶°ÇÑ »ç°í, ¼ÕÇØ¿¡ ´ëÇؼµµ ´ç»ç¿¡¼´Â Ã¥ÀÓÀ» ÁöÁö ¾Ê½À´Ï´Ù.
Keyword :
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ ÃֽŠapplication
Packaging Cell £¦ ½Ã½ºÅÛ
Retrovirus vector £¦ ½Ã½ºÅÛ
Çü±¤´Ü¹éÁú Retrovirus vector
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
Virus »óû¾×¿¡¼ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
Retrovirus ³óÃà
Receptor Boosters (Ecotropic and Amphotropic)
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analysis Kit