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Gene transfer (virus)
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Lentivirus
> Provirus Quantitation Kit
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy number Á¤·®
Provirus Quantitation Kit
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Clontech
631239
Lenti-X¢â Provirus Quantitation Kit
200 ȸ
(Package)
1,029,000¿ø
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,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
½ÇÇè °£ÀÇ transgene expression levelÀ» ¿¹Ãø °¡´É
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½ÇÇè °£ ¶Ç´Â ´Ù¸¥ ¼¼Æ÷ÁÖ °£ÀÇ ÀÏ°üµÈ copy number È®º¸
Genomic DNA purification kit 10ȸºÐ, TB Green qPCR premix 200ȸºÐ Æ÷ÇÔ
¸ñÀû¼¼Æ÷ÀÇ nuclear DNA¿¡ »ðÀÔµÈ lentivirusÀÇ ¾çÀ» Á¤·®ÇÔÀ¸·Î½á, Lentiviral supernatant stock ³»¿¡ Á¸ÀçÇÏ´Â ½ÇÁ¦ ¿ª°¡ (real titer, effective titer)¿Í MOI (Multiplicity of infection)¸¦ º¸´Ù Á¤È®ÇÏ°Ô ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù. À̸¦ ÅëÇÏ¿© ¾ó¸¶³ª ¸¹Àº ¹ÙÀÌ·¯½º À¯Àüü (viral genome)ÀÌ ¸ñÀû ¼¼Æ÷¿¡ »ðÀÔÀÌ µÇ¾ú´ÂÁö¸¦ ¿¹ÃøÇÒ ¼ö ÀÖ´Ù.
Lenti-X¢â Provirus Quantitation Kit´Â tranduced cell ³»ÀÇ »ðÀÔµÈ ·»Æ¼¹ÙÀÌ·¯½º (integrated lentivirus (provirus))ÀÇ copy number¸¦ ºü¸£°Ô Á¤·®ÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº genomic DNA purification kit¿Í 200ȸºÐÀÇ TB Green qPCR premix ¹×, calibrated control template¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù.
* º» Á¦Ç° ³»¿¡ Æ÷ÇÔµÈ primer´Â Packaging Signal¿¡ ÀÎÁ¢ÇÑ HIV-1 genome ¿µ¿ªÀ» ÁõÆøÇÒ ¼ö ÀÖµµ·Ï µðÀÚÀεǾî ÀÖ½À´Ï´Ù. º» ÇÁ¶óÀ̸Ӵ ´ÙÄ«¶ó¹ÙÀÌ¿À¿¡¼ ÆǸÅÇÏ´Â ¸ðµç Lentivirus vector »Ó¸¸ ¾Æ´Ï¶ó ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â HIV-1 based lentiviral vector¿¡ ȣȯ °¡´ÉÇÕ´Ï´Ù. ¸¸¾à, vector compatibilty¸¦ È®ÀÎÇÏ°íÀÚ ÇÑ´Ù¸é, °í°´Áö¿ø¼¾ÅÍ (TEL. 02-2081-2510)·Î ¿¬¶ô ¹Ù¶ø´Ï´Ù.
±×¸² 1. Measuring lentiviral copy number in target cells.
Provirus content is determined using a rapid qPCR method.
±×¸² 2. Lentiviral provirus copy number affects transgene expression levels in transduced cells.
HT1080 cells were infected at either high or low MOI using an AcGFP1 lentiviral expression vector. 72 hr after infection, the cells were subjected to puromycin drug selection. One week later, stably transduced clones were selected for expansion and analysis. AcGFP1 expression levels were determined using flow cytometry, and provirus content in genomic DNA was determined using the Lenti-X Provirus Quantitation Kit. The figure represents data pooled from two groups having either low copy number (>3; n = 8) or high copy number (>9; n = 7). Higher provirus contents were associated with higher expression levels.
±×¸² 3. Equivalent virus amounts in different cell types results in different provirus (integrated lentivirus) copy numbers.
Equivalent amounts of a single lentiviral stock (100 ¥ìl) were used to infect either HT1080, HeLa, and HEK 293 cells. 72 hr after infection, the cells were subjected to drug selection with puromycin. One week later, the provirus copy numbers of the stably transduced cell populations were determined using the Lenti-X Provirus Quantitation Kit.
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
Lenti-X Provirus Quantitation Components
NucleoSpin Tissue Kit (10 preps)
TB Green Advantage qPCR Premix (200 rxns)
°ü·ÃÁ¦Ç°
´Ü 5ºÐ, Instant Lentivirus Á¤·®:
Lenti-X¢â GoStix¢â Plus (Code 631280)
ºü¸£°í Á¤È®ÇÑ Lentivirus Á¤·®:
Lenti-X¢â qRT-PCR Titration Kit (Code 631235)
¹ÙÀÌ·¯½º ³óµµ°¡ ³·À» ¶§, ³óÃà:
Lenti-X¢â Concentrator (Code 631231)
Provirus »ðÀÔÀ§Ä¡ ºÐ¼®:
Lenti-X¢â Integration Site Analysis Kit (Code 631263)
Keyword : Lentivirus,virus,Lenti,·»Æ¼,·»Æ¼¹ÙÀÌ·¯½º,Á¤·®,¿ª°¡,titer,titration,provirus,ÇÁ·Î¹ÙÀÌ·¯½º,integrated virus,»ðÀÔ ¹ÙÀÌ·¯½º,moi
Lentivirus
[°³¿ä] Packaging Single Shots
[FAQ] Lentiviral Gene Delivery
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
ÀÓ»ó Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ
3¼¼´ë Lentivirus packaging system
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
ÃÖÀûÈµÈ Transfection Reagent & Packaging Mi..
Trans
IT¢ç Lentivirus System