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Gene transfer (virus)
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Retrovirus
> Retrovirus ³óÃà
Retrovirus ³óÃà
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Clontech
631455
Retro-X Concentrator
100 ml
494,000¿ø
Clontech
631456
Retro-X Concentrator
500 ml
1,706,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
ÃÊ°í¼Ó¿ø½ÉºÐ¸®±â ¾øÀÌ ¹ÙÀÌ·¯½º ¿ª°¡¸¦ 100 ¹è±îÁö Áõ°¡
°£´ÜÇÑ Á¶ÀÛ °úÁ¤ : mix, wait ±×¸®°í spin
¹ø°Å·Î¿ò ¾øÀÌ ºÎÇǸ¦ ½±°Ô scale up °¡´É
Retro-X Concentrator·Î retrovirusÀÇ ¿ª°¡¸¦ 100 ¹è±îÁö Áõ°¡½Ãų ¼ö ÀÖÀ¸¸ç, ´õ ¸¹Àº ¹ÙÀÌ·¯½º¸¦ ¸¸µé°Å³ª, Ãß°¡·Î packaging ¼¼Æ÷¿¡ transfectionÇÒ ÇÊ¿ä ¾øÀÌ ÃæºÐÈ÷ ³ôÀº MOI·Î
¸ñÀû¼¼Æ÷¸¦ °¨¿°½Ãų ¼ö ÀÖ´Ù.
Simple Á¶ÀÛ°úÁ¤: Mix, Wait ±×¸®°í Spin
Retro-X Concentrator´Â ºü¸£°í, °£ÆíÇÏ¸ç ¾î¶°ÇÑ Á¾·ùÀÇ retrovirus stockµµ ³óÃà½Ãų ¼ö ÀÖ´Â ¾ÆÁÖ È¿À²ÀûÀÎ ¹æ¹ýÀ» Á¦°øÇÑ´Ù. ¿ì¼± retrovirus »óÃþ¾×À» Retro-X Concentrator ½Ã¾à°ú ¼¯Àº ÈÄ ÇÏ·í¹ã ¹ÝÀÀ½ÃŲ ÈÄ ¹ÝÀÀ¾×À» ÀÏ¹Ý ¿ø½ÉºÐ¸®±â·Î ¿ø½ÉºÐ¸®ÇÑ´Ù (Figure 1). »óÃþ¾×ÀÇ ¾ç°ú viral envelope¿¡ µû¶ó MMLV ¶Ç´Â MSCV¿¡ ±â¹ÝÀ» µÐ retroivirusÀÇ ¿ª°¡¸¦
100 ¹è±îÁö Áõ°¡½Ãų ¼ö ÀÖÀ¸¸ç (Figure 2), ³ôÀº ȸ¼öÀ²À» ¾òÀ» ¼ö ÀÖ´Ù. ClontechÀÇ Retro-XSystemÀÇ ¸ðµç retroiviral vector »Ó¸¸ ¾Æ´Ï¶ó ´Ù¸¥ ¸ðµç retroivirusÀÇ »óÃþ¾×À»
Retro-X Concentrator·Î ³óÃàÇÒ ¼ö ÀÖÀ¸¸ç ÇÊ¿ä¿¡ µû¶ó ¿ë·®À» Áõ°¨ÇÒ ¼ö ÀÖ´Ù.
Table I: Retro-X Concentrator vs. Ultracentrifugation
Feature
Retro-X Concentrator
Ultracentrifugation
Easily Scalable
Yes
No
Specialized Equipment
No
Yes
Ease-of-Use
++++
+
100 ¹è±îÁö ¿ª°¡ Áõ°¡
Retro-X ConcentratorÀ» »ç¿ëÇÏ¸é ´Ù¾çÇÑ envelope-pseudotyped retroivirusÀÇ »óÃþ¾× 10 mlÀ» 100 ¥ìI·Î ³óÃàÇÏ¿© ¿ª°¡¸¦ 46~116 ¹è±îÁö Áõ°¡½Ãų ¼ö ÀÖ´Ù (Figure 2).
ÃÊ°í¼Ó¿ø½ÉºÐ¸®º¸´Ù ´õ¿í °£´ÜÇÑ ¹æ¹ý
Retro-X Concentrator°ú ÃÊ°í¼Ó¿ø½ÉºÐ¸® ¹æ¹ýÀ» ºñ±³ÇÏ¿´À» °æ¿ì, Retro -X ¹æ¹ýÀÇ ÀåÁ¡Àº ¸Å¿ì ¸íÈ®ÇÏ´Ù (Table 1). Retro-X Concentrator´Â ÃÊ°í¼Ó¿ø½ÉºÐ¸® ¹æ¹ýº¸´Ù ´õ ½±°í À¶Å뼺ÀÌ ÀÖ´Ù. Retro-X Concentrator ½Ã¾àÀº 4x ³óµµÀÇ ½Ã¾àÀ¸·Î Á¦°øµÇ±â ¶§¹®¿¡ »óÃþ¾×¿¡ Æ÷ÇÔµÈ ¹ÙÀÌ·¯½ºÀÇ ¾ç°ú ¿ª°¡¿¡ »ó°ü¾øÀÌ »ç¿ëÇÒ ¼ö ÀÖ´Ù. »óÃþ¾× 250 ml ±îÁöµµ Àû¿ë °¡´ÉÇÏ´Ù.
Figure 1. The Retro-X Concentrator protocol.
Add Retro-X Concentrator reagent to clarified viral supernatant, incubate overnight at 4¡ÆC, and spin. That¡¯s it.
Figure 2. Retro-X Concentrator increases virus titer by up to 100-fold
. Retrovirus expressing DsRed2 was packaged using the Retro-X Universal Packaging System and four different viral envelopes. Retro-X Concentrator was used to concentrate dualtropic (10A1), amphotropic (Ampho), ecotropic (Eco), and VSV-G (pantropic) pseudotyped retrovirus samples from 10 ml down to 100 ¥ìl (Panels A & B). Crude and concentrated viral stocks were then titrated on HT1080 or NIH 3T3 cells and analyzed via flow cytometry at 72 hr postinfection to determine the percentage of transduced cells.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Keyword :
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ ÃֽŠapplication
Packaging Cell £¦ ½Ã½ºÅÛ
Retrovirus vector £¦ ½Ã½ºÅÛ
Çü±¤´Ü¹éÁú Retrovirus vector
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
Virus »óû¾×¿¡¼ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
Retrovirus ³óÃà
Receptor Boosters (Ecotropic and Amphotropic)
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analysis Kit