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Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packaging vector mix

Packaging single shots

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631275
Lenti-X¢â Packaging Single Shots (VSV-G)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
16 ȸ
1,460,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32778
Clontech
631276
Lenti-X¢â Packaging Single Shots (VSV-G)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ
4,278,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
631282
Lenti-X¢â Packaging Single Shots (96-well, VSV-G)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
4 x 96-well plates
2,040,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x110886
Clontech
631277
Lenti-X¢â Packaging Single Shots (Integrase Deficient)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
16 ȸ
1,878,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32778
Clontech
631278
Lenti-X¢â Packaging Single Shots (Ecotropic)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
16 ȸ
1,878,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32778
Clontech
631294
Lenti-X¢â Packaging Single Shots (Envelope-Free)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
16 ȸ
(Package)
2,146,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â Lenti-X Packaging Single Shots (Envelope-Free) Protocol-at-a-Glance.pdf

  • 4¼¼´ëÀÇ lentiviral packaging systemÀ¸·Î, Æí¸®ÇÔ°ú ¼º´ÉÀ» ±Ø´ëÈ­ÇÑ ÇüÅÂ
  • ÀçÇö¼º ÀÖ°Ô ³ôÀº ¿ª°¡ (107-108 IFU/§¢)·Î virus packaging
  • °£´ÜÇÑ ÇÁ·ÎÅäÄÝ: Lentiviral DNA¸¦ ³Ö°í ¼¯Àº ÈÄ Àü¿ë packaging cell line¿¡ ÷°¡ÇÏ¸é ¹ÝÀÀ ¿Ï·á (´Ü, Envelope-free ŸÀÔÀÇ °æ¿ì, envelopeÀÇ cloning °úÁ¤ÀÌ ¿ä±¸µÊ)
  • °¢ tube ȤÀº well ³» transfection ½Ã¾à°ú lentiviral packaging vector, buffer°¡ ÃÖÀûÀÇ ºñÀ²·Î ºÐÁÖ ¹× µ¿°á°ÇÁ¶ µÇ¾î ÀÖ¾î, Ãß°¡ÀûÀÎ transfection ½Ã¾à Á¶°Ç °ËÅä ºÒÇÊ¿ä
  • Àû¿ëÇÏ°íÀÚ ÇÏ´Â ¼¼Æ÷, application¿¡ µû¶ó envelop type ¼±ÅÃ
Á¦Ç° ¼³¸í
Lenti-X¢â Packaging Single ShotsÀº 4¼¼´ë lentivirus packaging systemÀ¸·Î, ¾ÆÁÖ Æí¸®ÇÑ one-step ¹æ½ÄÀ¸·Î ½ÇÇèÀ» ÁøÇàÇϸç, Àü¿ë packaging cellÀ» ÀÌ¿ëÇßÀ» ¶§ ÀϹÝÀûÀ¸·Î 107-108 IFU/mlÀÇ °í¿ª°¡ Lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù.

Á¦Ç°Àº lentivirus¸¦ packaging ÇÒ ¼ö ÀÖ´Â vector¿Í transfection ½Ã¾àÀÌ ¸ðµÎ ÃÖÀûÀÇ ºñÀ²·Î single tube ³»¿¡ ºÐÁÖ ¹× µ¿°á°ÇÁ¶ µÇ¾î ÀÖ´Â ÇüÅ·Î, ¹ßÇöÀ» ¿øÇÏ´Â tropism (envelope)À» ¼±ÅÃÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, single tube ÇüÅÂ¿Í 96 well plate ÇüŸ¦ ¼±ÅÃÇÒ ¼ö ÀÖ¾î, high-throughputÀÌ ¿ä±¸µÇ´Â ½ÇÇè¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù.

Tropism (envelope)

Á¦Ç°¸í

Application

VSV-G

Lenti-X Packaging Single Shots (VSV-G)
Lenti-X Packaging Single Shots (96-well, VSV-G)

°ÅÀÇ ¸ðµç ¼¼Æ÷¿¡ °¨¿°·ÂÀ» °¡Á® ÀϹÝÀûÀ¸·Î »ç¿ëµÊ

Ecotropic

Lenti-X Packaging Single Shots (Ecotropic)

MLV ecotropic envelope glycoproteinÀ» °¡Áø mouse cell ȤÀº rat cell¿¡ À¯¿ë

Integrase deficient

Lenti-X Packaging Single Shots (Integrase Deficient)

Host cell genomeÀ¸·Î DNA integrationµÇ´Â È¿À²À» °¨¼Ò½ÃÄÑ ÀϽÃÀû ¹ßÇö À¯µµ

Envelop-free

Lenti-X Packaging Single Shots (Envelope-Free)

¿øÇÏ´Â envelope¸¦ Á÷Á¢ cloningÇÏ¿© »ç¿ë


  • VSV-G, ecotropic ¿ÜÀÇ tropismÀ» Àû¿ëÇßÀ» ¶§ ´õ ³ôÀº À¯ÀüÀÚ µµÀÔ È¿À²ÀÌ ±â´ëµÇ´Â °æ¿ì
  • Narrowed tropism¿¡¼­ ³ôÀº °¨¿° È¿À²À» º¸À̴ ƯÁ¤ÇÑ cell type Àû¿ë ½Ã
  • Envelope ¿¹) Baboon envelope, Cocal envelope, Measles envelope, Mokola envelope, Ebola envelope µî
  • Target cell ¿¹) Á¶Ç÷¸ð¼¼Æ÷ (hematopoietic cell), ¸é¿ª¼¼Æ÷ (immune cell - T cell, B cell µî), ƯÁ¤ Àå±â Ÿ°Ù

Lenti-X Packaging Single Shots (Envelope-Free)´Â single shot ³» envelope vector°¡ Æ÷ÇԵǾî ÀÖÁö ¾Ê°í, º°µµÀÇ empty vector ÇüÅ·ΠÁ¦°øµÈ´Ù. µû¶ó¼­ ¿øÇÏ´Â envelope protein ¼­¿­À» Á÷Á¢ cloningÇÏ°í, lentiviral expression vector¿Í ÇÔ²² single shot¿¡ ³Ö¾î virus¸¦ packagingÇÑ´Ù. ¶ÇÇÑ transduction È¿À²À» È®ÀÎÇÒ ¼ö ÀÖµµ·Ï ZsGreen1¿Í luciferase¸¦ °¢°¢ ¹ßÇöÇÏ´Â reporter vector 2Á¾ÀÌ Á¦Ç° ³» Æ÷ÇԵǾî ÀÖ´Ù.


±×¸² 1. The Lenti-X¢â Packaging Single Shots (VSV-G) protocol.
½ÇÇè ÁÖÀÇ»çÇ×
- 4¼¼´ëÀÇ lentivirus packaging systemÀº Tet-off ±â¼úÀÌ µµÀԵǾî ÀÖ¾î, packaging cell ¹è¾ç ½Ã tetracyclineÀ» Æ÷ÇÔÇÏÁö ¾Ê´Â ¹èÁö Á¶°ÇÀ¸·Î ¹è¾çÇØ¾ß ÇÑ´Ù. Tetracycline-derived contaminants·Î ÀÎÇÑ background¸¦ ÃÖ¼ÒÈ­ÇÒ ¼ö ÀÖ´Â Tet approved FBSÀÇ »ç¿ëÀ» Àû±Ø ±ÇÀåÇÑ´Ù.
- Á¦Ç° ³»¿¡´Â packaging cell lineÀ» Æ÷ÇÔÇÏ°í ÀÖÁö ¾Ê´Ù. º¸´Ù °í¿ª°¡ÀÇ ¹ÙÀÌ·¯½º¸¦ »ý»êÇÒ ¼ö ÀÖ´Â Lenti-X¢â 293T Cell Line (Code 632180)¸¦ ÇÔ²² ±¸¸ÅÇÏ¿© »ç¿ëÇÒ °ÍÀ» ÃßõÇÑ´Ù.
Applications
- ´Ù¾çÇÑ envelopeÀ» °¡Áø Lentivirus »ý»ê
- Transfection È¿À²ÀÌ ³·Àº ¼¼Æ÷ ³» À¯ÀüÀÚ µµÀÔ
- Stable cell line ±¸Ãà
- Lentivirus¸¦ ÀÌ¿ëÇÑ CRISPR ȤÀº shRNA screening, vector optimizationÀÇ HTP ºÐ¼®
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
* Lenti-X¢â Packaging Single Shots (VSV-G) (Code 631275)
   1 box containing 16 Lenti-X¢â Packaging Single Shots (VSV-G), in a foil pouch

* Lenti-X¢â Packaging Single Shots (VSV-G) (Code 631276)
   1 box containing 96 Lenti-X¢â Packaging Single Shots (VSV-G), in a foil pouch

* Lenti-X¢â Packaging Single Shots (96-well, VSV-G) (Code 631282)
   4 x Lenti-X Packaging Single Shots 96-well Plate (VSV-G) in foil pouches

* Lenti-X¢â Packaging Single Shots (Ecotropic) (Code 631277)
   1 box containing 16 Lenti-X Packaging Single Shots (Ecotropic), in a foil pouch

* Lenti-X¢â Packaging Single Shots (Integrase deficient) (Code 631278)
   1 box containing 16 Lenti-X¢â Packaging Single Shots (Integrase Deficient), in a foil pouch

* Lenti-X Packaging Single Shots (Envelope-Free) (Code 631294)
   Lenti-X Packaging Single Shots (Envelope-Free)
   pEmpty Vector
   Lenti-X Reporter Vector Set




Keyword : Lentivirus,Lenti-virus,packaging,Lentiviral,Lentivral delivery,Lenti,soeasy,so easy

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>
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>
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
>
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Packaging single shots
>
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
>
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
>
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
>
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
>
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
>
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
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Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
>
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
>
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
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¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
>
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
>
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
>
ÃÖÀûÈ­µÈ Transfection Reagent & Packaging Mi..
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