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°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vector

pMEI-5 DNA

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(ºÎ°¡¼¼º°µµ)
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Takara
3655
pMEI-5 Neo DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
511,200¿ø 
639,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3653_3658_e.v2008Da.pdf
Takara
3656
pMEI-5 DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
511,200¿ø 
639,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3653_3658_e.v2008Da.pdf

Á¦Ç°¼³¸í
pMEI-5 DNA´Â °í¹ßÇö À¯ÀüÀÚ µµÀÔ¿ë retrovirus vectorÀÌ´Ù. º» vector´Â packaging cell line¿¡ transfection½ÃÄÑ ÀϽÃÀû ȤÀº genome¿¡ integrationµÇ¾î ¹ÙÀÌ·¯½º packaging signal(¥÷), ¸ñÀû À¯ÀüÀÚ, selection marker¸¦ Æ÷ÇÔÇÑ Àü»ç »ê¹°À» ¾ÈÁ¤ÀûÀ¸·Î ¹ßÇö½Ãų ¼ö ÀÖ´Ù. LTR°ú packaging signal(¥÷), ÀÌ¿ÜÀÇ MoMLV À¯·¡ À¯ÀüÀÚ(gag, pol, env)¸¦ Æ÷ÇÔÇÏÁö ¾Ê°í ÀÖÀ¸¸ç, splicing ´É·ÂÀÌ ³ôÀº intronÀ» ÀÌ¿ëÇÏ¿© ³ôÀº Àü»ç´ÉÀ» °®°í ÀÖ´Ù´Â °ÍÀÌ Æ¯Â¡ÀÌ´Ù. º» vector¸¦ ÀÌ¿ëÇϸé, pDON-AI-2 series¿¡ ºñÇØ ¾à 2~8¹è ³ôÀº À¯ÀüÀÚ ¹ßÇöÀÌ °¡´ÉÇϹǷÎ, µµÀÔ À¯ÀüÀÚ¸¦ °í¹ßÇöÇÏ°íÀÚ ÇÒ ¶§ À¯¿ëÇÏ´Ù. pMEI-5 Neo DNA (Code 3655)´Âselection marker·Î neomycin resistance geneÀ» °¡Áö°í ÀÖ´Ù.
º¸Á¸ -20¡É
Çü»ó
10 mM Tris-HCl (pH8.0)
1 mM EDTA
±æÀÌ
pMEI-5 Neo DNA £º5,820 bp
pMEI-5 DNA £º4,689 bp
License Notice : [L21]
pMEI-5 DNAÀÇ ±¸Á¶

Keyword :

Retrovirus
>
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
>
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
>
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
>
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
>
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
>
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
>
Virus »óû¾×¿¡¼­ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
>
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤