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À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ¿¬±¸ÀÚ¿¡°Ô ±ÇÀå

pDON-5 DNA

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
3657
pDON-5 Neo DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
511,200¿ø 
639,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3653_3658_e.v2008Da.pdf
Takara
3658
pDON-5 DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
511,200¿ø 
639,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3653_3658_e.v2008Da.pdf

º¸Á¸ -20¡É
Á¦Ç°¼³¸í
º» º¤ÅÍ´Â LTR°ú packaging signal (¥× ¼­¿­) ÀÌ¿ÜÀÇ MoMLV À¯·¡ À¯ÀüÀÚ (gag, pol, env)¸¦ Æ÷ÇÔÇÏÁö ¾Ê°í, cloning site »óºÎ¿¡ splicing ´É·ÂÀÌ ³ôÀº Àΰ£ EF1¥áÀ¯·¡ÀÇ ÀÎÆ®·Ð (intron)À» Æ÷ÇÔÇÏ¿© ¸ñÀûÀ¯ÀüÀÚ¸¦ ³ôÀº È¿À²·Î ¹ßÇö½ÃÅ°´Â °ÍÀÌ Æ¯Â¡ÀÌ´Ù. ¶ÇÇÑ 5¡¯ LTRÀÇ U3 ¿µ¿ªÀÌ º¸´Ù °­·ÂÇÑ cytomegalovirus À¯·¡ÀÇ promoter·Î ġȯµÇ¾î ÀÖ¾î °í¿ª°¡ÀÇ ÀçÁ¶ÇÕ retrovirus¸¦ ¾òÀ» ¼ö ÀÖ´Ù. µû¶ó¼­ º» vector´Â pMEI-5½Ã¸®Á´Ù °í¿ª°¡ÀÇ ÀçÁ¶ÇÕ retrovirus¸¦ ¾òÀ» ¼ö ÀÖ°í, pDON-AI-2 ½Ã¸®Áî¿¡ ºñÇØ ³ôÀº À¯ÀüÀÚ ¹ßÇöÀ» ±â´ëÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, pDON-5 Neo DNA (Code 3657)Àº ¼±º° ¸¶Ä¿·Î neomycin³»¼º À¯ÀüÀÚ¸¦ °¡Áö°í ÀÖ´Ù.
Çü»ó
10 mM Tris-HCl (pH8.0) 1 mM EDTA
±æÀÌ
pDON-5 Neo DNA : 5,828 bp
pDON-5 DNA : 4,697 bp
pDON-5 Neo DNAÀÇ ±¸Á¶

Keyword :

Retrovirus
>
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
>
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
>
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
>
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
>
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
>
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
>
Virus »óû¾×¿¡¼­ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
>
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤