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239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿À²

Packaging Cell Lines

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
632180
Lenti-X¢â 293T Cell Line
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
1 ml
737,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32761

[ÁÖÀÇ] µ¿°áµÈ ¼¼Æ÷ÁÖÀÇ Çص¿(Thawing) ½Ã, ¼¼Æ÷ »ýÁ¸À² Çâ»óÀ» À§ÇÏ¿© collagen-coated plate ¶Ç´Â flaks¸¦ »ç¿ëÇϽʽÿÀ.
         ÃßÈÄ ¼¼Æ÷ ¹è¾ç ½Ã¿¡µµ ¼¼Æ÷ÁÖÀÇ ºÎÂøÀ²(adherence)ÀÌ ÁÁÁö ¾Ê´Ù¸é collagen-coated plate ¶Ç´Â flask¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.

NOTE: We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates (e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.


  • °í¿ª°¡ÀÇ ·»Æ¼¹ÙÀÌ·¯½º Á¦ÀÛÀ» À§ÇØ Á¦ÀÛµÈ 293T Clone
  • 293FT Cell LineÀÇ 6¹è, HEK293 Cell Line¿¡ ºñÇÏ¿© 30¹è ¿ª°¡°¡ ³ôÀº ¹ÙÀÌ·¯½º »ý»ê
  • ´Ù¾çÇÑ Á¾·ùÀÇ ·»Æ¼¹ÙÀÌ·¯½º º¤ÅÍ¿¡ Àû¿ë °¡´É
  • Lenti-X¢â Packaging single shots (Code 631275)¿Í ÇÔ²² »ç¿ëÇϸé 10^8 IFUÀÇ ¹ÙÀÌ·¯½º¸¦ Á¦ÀÛ °¡´É (10cm plate ±âÁØ)
Á¦Ç°¼³¸í
Lenti-X¢â 293T Cell LineÀº °í¿ª°¡ÀÇ ·»Æ¼¹ÙÀÌ·¯½º¸¦ Á¦ÀÛ °¡´ÉÇÑ ·»Æ¼¹ÙÀÌ·¯½º ÆÐŰ¡¿ë ¼¼Æ÷ÁÖÀÌ´Ù. º» ¼¼Æ÷ÁÖ¸¦ ÀÌ¿ëÇϸé ÀϹÝÀûÀ¸·Î »ç¿ëÇÏ´Â 293FT Cell Line°ú HEK293 Cell LineÀ» ÀÌ¿ëÇÏ¿´À» ¶§º¸´Ù ¾à 6¹è-30¹è ¿ª°¡°¡ ³ôÀº ¹ÙÀÌ·¯½º¸¦ ¾òÀ» ¼ö ÀÖ´Ù (±×¸² 1)


±×¸² 1. Lenti-X¢â 293T Cell LineÀÇ ¶Ù¾î³­ ¹ÙÀÌ·¯½º »ý»ê ´É·Â
We used our 4th generation lentiviral packaging system and one of our pLVX lentiviral vectors to compare the virus production of the Lenti-X 293T Cell Line to that of two other commonly used HEK 293-based cell lines. Lenti-X 293T cells clearly outperformed the other cell lines?producing over 6 times more virus than 293FT cells and up to 30 times more virus than the parental HEK 293 cell line.
½Ã³ÊÁö È¿°ú : ³ôÀº ¿ª°¡¸¦ À§ÇÑ ÇØ´ä
¾Æ·¡ÀÇ ±¸¼º¿ä¼Ò¸¦ 293T ¼¼Æ÷¿Í ÇÔ²² »ç¿ëÇϸé ClontechÀÇ Lenti-X System Vector »Ó¸¸ ¾Æ´Ï¶ó ´Ù¸¥ lentivirus vector¸¦ ÀÌ¿ëÇÒ ¶§µµ ÃÖ°í ¿ª°¡ÀÇ ¾ÈÀüÇÑ lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù.
  • ÃÖÀûÈ­µÈ Packaging Mix - ClontechÀÇ 4¼¼´ë packaging mixÀÎ Lenti-X¢â Packaging Single Shots´Â °í¿ª°¡ÀÇ Lentivirus¸¦ °£´ÜÇÏ°í È¿À²ÀûÀ¸·Î Á¦ÀÛÇÒ ¼ö ÀÖµµ·Ï ÃÖÀûÈ­ µÇ¾î ÀÖ´Ù. °¢°¢ÀÇ packaging vector¿Í transfection ½Ã¾àÀÌ ¸ðµÎ ÃÖÀûÀÇ ºñÀ²·Î È¥ÇÕµÇ¾î µ¿°á°ÇÁ¶ »óÅ·ΠÁ¦°øµÇ¸ç, lentiviral expression vectorÀÇ Ã·°¡¸¸À¸·Î ¾ÆÁÖ ¼Õ½±°Ô packagingÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
  • Tet System Transactivation - Tetracycline-responsive promoter element(TRE)ÀÇ Tet-Off transactivationÀ» ÅëÇÏ¿© packaging¿¡ ÇÊ¿äÇÑ ¸î °¡Áö ÁÖ¿ä ±¸¼º¿ä¼ÒµéÀ» °íµµ·Î ¹ßÇö½ÃŲ´Ù. HEK293 ±â¹ÝÀÇ cell lines¿¡¼­ Tet System À¯µµ¹ßÇöÀº CMV promoter¿¡ ÀÇÇØ »ý»êµÇ´Â ´Ù¸¥ ½Ã½ºÅÛ¿¡¼­ º¸´Ù ÈξÀ ³ôÀº ¹ßÇöÀ» À̲ø¾î³½´Ù (1).
  • ÃÖÀûÈ­µÈ transfection - xfect´Â °íÈ¿À²ÀÇ ºñµ¶¼º transfectionÀ» °¡´ÉÇÏ°Ô Çϸç, lipid-based transfection ¹ýº¸´Ù ´õ ³ôÀº ¹ßÇöÀ» À¯µµÇÑ´Ù. Lentiphos HT´Â 293T packaging cells¿¡ ÃÖÀûÈ­µÇ¾î ÀÖÀ¸¸ç, 99% ÀÌ»óÀÇ transfection È¿À²À» ¾òÀ» ¼ö ÀÖ´Ù. ÃÖÀûÈ­µÈ pH¿Í buffer Á¶¼º, ¿°³óµµ, ¹ÝÀÀ ¿ë·®°ú ÇÔ²² °£´ÜÇÑ ½ÇÇè Á¶ÀÛ °úÁ¤Àº ÃÖÀûÀÇ transfectionÀ» º¸ÀåÇÑ´Ù.
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº COA¸¦ ÂüÁ¶Çϼ¼¿ä)
1ml Lenti-X 293T Cell Line (2.0 x 106 cells/tube)
º¸Á¸
Liquid nitogren (-196 ¡É)
¶Ç´Â Á¦Ç° ¼ö·É ÈÄ, ¹Ù·Î À¶ÇØ ¹× ¹è¾ç ±ÇÀå
Shipping
Dry ice (-80 ¡É)
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
References
Yin, D. X. et al. (1996) Anal. Biochem. 235:195-201.




Keyword :

Lentivirus
>
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
>
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
>
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
>
ÀÓ»ó Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ
3¼¼´ë Lentivirus packaging system
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´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
>
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
>
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
>
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
>
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
>
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
>
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
>
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
>
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
>
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
>
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
>
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
>
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
>
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
>
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
>
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
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ÃÖÀûÈ­µÈ Transfection Reagent & Packaging Mi..
TransIT¢ç Lentivirus System