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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Lentivirus > (Legacy) Tet-Inducible expression ½Ã½ºÅÛ
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â ½Ã½ºÅÛ

(Legacy) Tet-Inducible expression ½Ã½ºÅÛ

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
631101
Tet System Approved FBS, US-Sourced
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ml
1,776,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
632163
Lenti-X¢â Tet-Off¢ç Advanced Inducible Expression System
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Set
(Package)
3,290,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33474, x32766, x32768, x32774, x32784, x33200
Clontech
631106
Tet System Approved FBS
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ml
1,739,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
631311
Doxycycline
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 g
376,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x94899, x32770, x32771

¢º º» Á¦Ç°Àº 2¼¼´ë ¹öÀüÀÇ Tetracycline-inducible expression system ÀÔ´Ï´Ù. º¸´Ù ´õ ¾÷±×·¹À̵åµÈ 3¼¼´ë ¹öÀü Tet-On 3G Á¦Ç°À» ¸¸³ª¼¼¿ä!
Lenti-X Tet-On 3G Inducible Expression System (Ŭ¸¯)

  • ³·Àº basal expression°ú ÃÖ°íÀÇ ¹ßÇö¿ª°¡¸¦ Á¤È®ÇÏ°Ô Á¶Àý
  • Lenti-X Packaging Single Shots (VSV-G) Æ÷ÇÔ: °íÈ¿À², ¾ÈÁ¤¼º ³ôÀº ·»Æ¼¹ÙÀÌ·¯½º ÆÐŰ¡°ú »ý»ê
  • Lenti-X Tet-On Advanced¿Í Lenti-X Tet-Off Advanced´Â ´Ù¾çÇÑ cell lines, ºñºÐ¿­¼¼Æ÷, Áٱ⼼Æ÷ ¹× Á¶Á÷¿¡ inducible gene expression systemÀ» ±¸Ãà
±âÀÛ
DoxycyclineÀ¸·Î Á¶ÀýµÇ´Â ¡±transactivators¡±(Tet-On°ú Tet-Off Advanced)´Â 3 °³ÀÇ ÀÛÀº HSV VP16 ´Ü¹éÁúÀÇ minimal transcription activation domain°ú TetR-À¯·¡ÀÇ DNA-binding domainÀÌ ¿¬°áµÈ À¶ÇÕ ´Ü¹éÁúÀÌ´Ù.
Transactivators´Â ³ôÀº ƯÀ̼ºÀ¸·Î Tetracycline Response Element (TRE)¿Í °áÇÕÇÑ´Ù. TRE´Â PLVX-Tight-PuroÀÇ PTight promoter¿¡ À§Ä¡ÇÏ°í ÀÖÀ¸¸ç ½ÇÇèÀÚÀÇ ¸ñÀû À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÑ´Ù. ½Ã½ºÅÛÀÌ À¯µµµÇ¸é PTight ·ÎºÎÅÍ ¸ñÀû À¯ÀüÀÚ°¡ ³ôÀº ¼öÁØÀ¸·Î Àü»çµÈ´Ù.
Tet-Off Advanced SystemÀº Dox°¡ ÀÖÀ» ¶§ basal state°¡ À¯ÁöµÇ°í, Dox°¡ Á¦°ÅµÇ¸é Àü»ç°¡ ÁøÇà µÈ´Ù. ¹Ý´ë·Î Tet-On Advanced SystemÀº Dox°¡ ÀÖÀ» ¶§ Àü»ç°¡ È°¼ºÈ­µÈ´Ù (Figure 1).


Figure 1. The Lenti-X Tet-Advanced Systems are highly inducible. Using equal amounts of high-titer supernatants, HeLa cells cultured at the indicated concentrations of Dox were cotransduced for 8 hr with an LVXTight-Puro-Luc lentivirus and an LVX-Tet-On Advanced lentivirus. Cultures were harvested after 48 hr and assayed for luciferase activity. Luciferase was expressed at very high levels, while the basal/uninduced expression level was very low.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.




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