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Gene transfer (virus)
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Retrovirus
> Retrovirus Packaging Kit
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovirus ÀÔÀÚ¸¦ »ý»ê
Retrovirus Packaging Kit
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Takara
6160
Retrovirus Packaging Kit Eco
10 ȸºÐ
336,800¿ø
421,000¿ø
09.02 ~ 10.31
Takara
6161
Retrovirus Packaging Kit Ampho
10 ȸºÐ
336,800¿ø
421,000¿ø
09.02 ~ 10.31
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
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º» Á¦Ç°Àº ¸ñÀû À¯ÀüÀÚ¸¦ µµÀÔÇÑ ÀçÁ¶ÇÕ retrovirus vector 2Á¾·ù¿Í packaging vector¸¦ Àλê Ä®½·¹ý¿¡ ÀÇÇØ µ¿½Ã¿¡ ¼¼Æ÷·Î µµÀÔÇÏ¿© °í¿ª°¡ÀÇ ÀçÁ¶ÇÕ retrovirus ÀÔÀÚ¸¦ »ý»êÇÒ ¼ö ÀÖ´Â kitÀÌ´Ù.
Kit¿¡´Â packaging vector·Î
gag-pol
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env
¹ßÇö vector (ecotropic ¶Ç´Â amphotropic)°¡ Æ÷ÇԵǾî ÀÖ¾î rat, mouse ¼¼Æ÷¿¡ °¨¿° °¡´ÉÇÑ ecotropic virus¸¦ »ý»êÇÏ´Â Retrovirus Packaging Kit Eco¿Í ´Ù¼öÀÇ Æ÷À¯µ¿¹°¼¼Æ÷¿¡ °¨¿°°¡´ÉÇÑ amphotropic virus¸¦ »ý»êÇÏ´Â Retrovirus Packaging Kit Ampho µÎ Á¾·ù°¡ ÀÖ´Ù.
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(packaging signal)¿Í LTR ¼¿À» °¡Áö´Â ÀçÁ¶ÇÕ retrovirus vector º» plasmid¿Í packaging vector ±×¸®°í ÷ºÎµÈ transfection ½Ã¾àÀ» ÀÌ¿ëÇϸé 293 ¼¼Æ÷ µî¿¡ ÇÔ²² µµÀÔÇÏ¿© 48 ½Ã°£ ÈÄ ÀϽÃÀûÀ¸·Î °í¿ª°¡ (105~107 cfu/ ml)ÀÇ ÀçÁ¶ÇÕ virus ÀÔÀÚ¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù.
Packaging vector´Â virus ÀÔÀÚ Çü¼º°ú º¹Á¦¿¡ ÇÊ¿äÇÑ retrovirusÀÇ ±¸Á¶ À¯ÀüÀÚÀÎ
gag, pol
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ÀÌ¿Ü¿¡´Â retrovirus À¯·¡ÀÇ ¼¿À» ÀüÇô Æ÷ÇÔÇÏÁö ¾ÊÀ¸¹Ç·Î ÀçÁ¶ÇÕ retrovirus vector¿Í ÀçÁ¶ÇÕ¿¡ ÀÇÇØ Àڱ⺹Á¦´É·ÂÀ» °¡Áø retrovirusÀÇ »ý»ê °¡´É¼ºÀÌ ¸Å¿ì ³·´Ù.
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pGP Vector (1 §¶/ §¡)
50 §¡
pE-eco (¶Ç´Â ampho) Vector (1 §¶/ §¡)
50 §¡
Transfection Buffer
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500 §¡ ¡¿ 10
2 M CaCl
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620 §¡
25 mM Chloroquine
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40 §¡
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ÀçÁ¶ÇÕ RetrovirusÀÇ Á¦ÀÛ °³¿ä
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(¶ÇÇÑ º» Á¦Ç°¿¡ ÀÇÇØ È¹µæÇÑ »ý¹°Àç·á´Â Á¦ 3ÀÚ¿¡°Ô ¾çµµÇÒ ¼ö ¾ø½À´Ï´Ù.) ¶ÇÇÑ, ½ÄÇ°, ÈÀåÇ°, °¡Á¤¿ëÇ° µîÀ¸·Î »ç¿ëÇÒ ¼ö ¾ø½À´Ï´Ù.
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º» retrovirus·Î »ý¼ºµÈ virus »óÃþÀº »ðÀÔ´ÜÆí¿¡ ÀÇÇÑ À§ÇèÇÑ ¹ÙÀÌ·¯½º°¡ ÇÔÀ¯µÇ¾î ÀÖÀ¸¹Ç·Î ÀçÁ¶ÇÕ retrovirus »ý»ê°ú Ãë±Þ¿¡´Â ÀûÀýÇÑ Ã³¸®¸¦ ÃëÇØ¾ß ÇÕ´Ï´Ù. »ç¿ë½Ã¿¡´Â °üÇÒ °üû¿¡¼ Á¤ÇÑ ÀçÁ¶ÇÕ DNA ½ÇÇèÁöħ¿¡ µû¶ó ½ÇÇèÇϽñ⠹ٶø´Ï´Ù.
º» Á¦Ç°ÀÇ »ç¿ë°ú °ü·ÃµÈ »ç°í, ¼ÕÇØ¿¡ ´ëÇؼ ´ç»ç¿¡¼ Ã¥ÀÓÀ» ÁöÁö ¾ÊÀ¸¹Ç·Î ÁÖÀÇÇÏ¿© »ç¿ëÇϽñ⠹ٶø´Ï´Ù.
Keyword :
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ ÃֽŠapplication
Packaging Cell £¦ ½Ã½ºÅÛ
Retrovirus vector £¦ ½Ã½ºÅÛ
Çü±¤´Ü¹éÁú Retrovirus vector
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
Virus »óû¾×¿¡¼ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
Retrovirus ³óÃà
Receptor Boosters (Ecotropic and Amphotropic)
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analysis Kit