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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Adeno-Associated Virus (AAV) > AAVpro¢ç Titration Kit (for Real Time PCR)
ºü¸£°í Á¤È®ÇÑ AAV ¿ª°¡ÃøÁ¤ (qPCR)

AAVpro¢ç Titration Kit (for Real Time PCR)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
6233
AAVpro¢ç Titration Kit (for Real Time PCR)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
100 ȸ
584,000¿ø 
730,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6233_e.v2001.pdf

  • ´Ù¾çÇÑ serotypeÀÇ AAV¸¦ Á¤·®
  • ºü¸£°í °£ÆíÇÏ°Ô ¾à5½Ã°£¸¸¿¡ AAV Á¤·®
  • ÀϹÝÀûÀÎ DNA blot ¶Ç´Â ELISA ¹æ¹ý ´ëºñ Á¤È®ÇÑ titration ÃøÁ¤
  • Packaging cell line¿¡¼­ AAV ÃßÃâ, Real Time PCR±îÁö ÇÊ¿äÇÑ ¸ðµç ±¸¼ºÇ° Æ÷ÇÔ
Á¦Ç° ¼³¸í
Adeno-Associated Virus (AAV)´Â ºÐ¿­ ¹× ºñºÐ¿­ ¼¼Æ÷¿¡ ¸ðµÎ Àû¿ëÀÌ °¡´ÉÇϸç, ƯÈ÷ ºñºÐ¿­ ¼¼Æ÷¿¡¼­ Àå±â°£ À¯ÀüÀÚ ¹ßÇöÀÌ °¡´ÉÇÏ´Ù. ¶ÇÇÑ ¸é¿ª¿ø¼º (immune response)ÀÌ ³·¾Æ in-vivo ½ÇÇè¿¡ ÃÖÀûÈ­µÈ ¹ÙÀÌ·¯½ºÀÌ´Ù.
AAVÀÇ ¿ª°¡ÃøÁ¤Àº °¨¿° Á¶Á÷ ¹× °¨¿° ¹æ¹ý¿¡ ÃÖÀûÀÎ AAVÀÇ ¾çÀ» °áÁ¤ÇÏ°í ÀÏ°üµÈ ½ÇÇè °á°ú¸¦ µµÃâÇϱâ À§ÇØ ¸Å¿ì Áß¿äÇÏ´Ù. AAVpro® Titration Kit (for Real Time PCR)Àº ´ÙÄ«¶ó°¡ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ Real Time PCR¹æ½ÄÀÇ titration Kit·Î ºü¸£°í È¿À²ÀûÀ¸·Î AAVÀÇ ¿ª°¡¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù.

ÀÌ Á¦Ç°¿¡´Â AAV packaging cell line¿¡¼­ AAV¸¦ ÃßÃâÇÏ´Â °úÁ¤ºÎÅÍ Real Time PCR Á¤·®À» À§ÇÑ ½Ã¾àÀÌ ¸ðµÎ Æ÷ÇԵǾî ÀÖ´Ù. AAV vector ¾ç ¸»´Ü¿¡ Á¸ÀçÇÏ´Â ITR (Inverted Terminal Repeat)À» targetÀ¸·Î AAV genomeÀ» °ËÃâÇϱ⠶§¹®¿¡, ±âÁ¸ÀÇ DNA blot ¶Ç´Â ELISA ¹ý¿¡ ºñÇØ ºü¸£°í Á¤È®ÇÑ °á°ú¸¦ ¾òÀ» ¼ö ÀÖÀ¸¸ç, ´Ù¾çÇÑ AAV serotype*1¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
*1 AAV¿¡¼­ ÀϹÝÀûÀ¸·Î »ç¿ëÇÏ´Â AAV2À¯·¡ÀÇ ITR ¼­¿­À» °ËÃâÇϹǷÎ, ITR¿µ¿ªÀÌ AAV2·ÎºÎÅÍ À¯·¡ÇÑ ¼­¿­À̸é serotype¿¡ °ü°è¾øÀÌ Á¤·® °¡´ÉÇÏ´Ù.


±×¸² 1. AAVpro® Titration Kit (for Real Time PCR) workflow


±×¸² 2. ´Ù¾çÇÑ serotype¿¡¼­ AAVpro® Titration Kit (for Real Time PCR) Àû¿ë¼º È®ÀÎ
ITR ¼­¿­ (AAVpro® Titration Kit (for Real Time PCR)¿¡¼­ »ç¿ë)°ú, CMVÀÇ ¼­¿­À» Ÿ°ÙÇÏ´Â °¢°¢ÀÇ primer setÀ» »ç¿ëÇØ AAV1, 2, 6ÀÇ ¼¼ Á¾·ùÀÇ ¹ÙÀÌ·¯½º¿¡ ¿ª°¡¸¦ ÃøÁ¤ÇÏ¿´´Ù. ±× °á°ú ¼¼ Á¾·ùÀÇ serotype ¸ðµÎ CMV¸¦ Ÿ°ÙÇÑ °á°ú¿Í À¯»çÇÑ °æÇâÀ» º¸¿© AAVpro® Titration Kit°¡ AAV2À¯·¡ÀÇ ITR ¼­¿­À» °¡Áø ´Ù¾çÇÑ serotypeÀÇ AAV Á¤·®¿¡ ÀÌ¿ë °¡´ÉÇÏ´Ù´Â °ÍÀ» È®ÀÎÇß´Ù.
³»¿ë
AAVpro¢ç Titration Kit (for Real Time PCR) (Code 6233, 100 È¸)
1.

AAV ÃßÃ⠽þà

 

 

AAV Extraction Solution A

2 x 1.5 §¢

 

AAV Extraction Solution B

300 §¡

2.

AAV genome °£ÀÌ ÃßÃâ (100 ȸ)

 

 

DNase I

100 §¡

 

10×DNase I Buffer

200 §¡

 

Lysis Buffer

2 x 1 §¢

3.

Real Time PCR (25 §¡ ¹ÝÀÀ ½Ã, 100ȸ)

 

 

TB Green™ Premix Ex Taq II (Tli RNaseH Plus)

2 x 625 §¡

 

AAV Forward Titer Primer

20 §¡

 

AAV Reverse Titer Primer

20 §¡

 

dH2O

3 x 1 §¢

 

ROX Reference Dye

50 §¡

 

ROX Reference Dye II

50 §¡

 

Positive Control

50 §¡

 

EASY Dilution (for Real Time PCR)

5 x 1 §¢

º¸Á¸
TB Green® Premix Ex Taq II (Tli RNaseH Plus): 4¡É, Â÷±¤ (Àå±â º¸Á¸½Ã -20¡É, À¶ÇØ ÈÄ¿¡´Â 4¡É º¸Á¸)
±× ¿Ü: -20 ¡É (AAV Extraction Solution A, B, Lysis Buffer´Â À¶ÇØ ÈÄ´Â ½Ç¿Â º¸Á¸)
°ü·ÃÁ¦Ç°

Keyword : Adeno Associated Virus,in vivo,aav virus

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