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°£ÆíÇÑ AAV ÃßÃâ½Ã¾à

Extraction Solution

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
6235
AAVpro¢ç Extraction Solution
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Set
176,000¿ø 
220,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6235_e.v1509Da.pdf

  • Freeze/thaw, sonicationÀÌ ÇÊ¿ä¾ø´Â ÃÊ °£´Ü ÃßÃâ¹ý
  • AAV producing cell¿¡ ½Ã¾àÀ» ÷°¡ÇÏ¿© ¿ø½ÉºÐ¸®·Î AAV ÃßÃâ ¿Ï·á
  • ´Ù¾çÇÑ serotypeÀÇ AAV¸¦ °íÈ¿À²·Î ÃßÃâ °¡´É
  • CellÀ¯·¡ ´Ü¹éÁúÀ̳ª ÇÙ»êÀÇ È¥ÀÔ ³·Àº ÃßÃâ¹ý
Á¦Ç° ¼³¸í
Adeno-Associated Virus (AAV)´Â ºÐ¿­, ºñºÐ¿­ ¼¼Æ÷¿¡ À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇϸç, ƯÈ÷ ºñºÐ¿­ ¼¼Æ÷¿¡ ´ëÇØ Àå±â°£ À¯ÀüÀÚ ¹ßÇöÀÌ °¡´ÉÇϸç ƯÈ÷, ¸é¿ª¿ø¼º (immune response)ÀÌ ³·¾Æ in-vivo ½ÇÇè¿¡ ÃÖÀûÈ­µÈ virusÀÌ´Ù.

ÀϹÝÀûÀ¸·Î Freeze/thaw¹æ¹ý ¶Ç´Â sonication µîÀ» ÅëÇØ AAV producing cell·ÎºÎÅÍ AAV¸¦ ÃßÃâÇÏÁö¸¸, ÀÌ·¯ÇÑ ¹æ¹ýÀº ½Ã°£ÀÌ ¸¹ÀÌ ¼Ò¿äµÇ°í ¹ø°Å·Î¿ì¸ç Ư¼öÇÑ Àåºñ¸¦ ÇÊ¿ä·Î ÇÏ´Â °æ¿ìµµ ÀÖ´Ù.
º» Á¦Ç°Àº ´ÙÄ«¶ó¿¡¼­ µ¶ÀÚ °³¹ßÇÑ AAV extraction solution À¸·Î, µ¿°áÀ¶Çظ¦ ¹Ýº¹Çϰųª sonication µîÀÇ ¹ø°Å·Î¿î Á¶ÀÛ¾øÀÌ AAV producing cell¿¡ ½Ã¾àÀ» ÷°¡ÇÑ ÈÄ ¿ø½ÉºÐ¸®ÇÏ´Â ¹æ¹ýÀ¸·Î °£ÆíÇϸ鼭µµ È¿À²ÀûÀ¸·Î AAV¸¦ ÃßÃâÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°À» ÀÌ¿ëÇÏ¿© ÃßÃâÇÑ AAV´Â ¼÷ÁÖÀ¯·¡ ´Ü¹éÁúÀ̳ª ÇÙ»êÀÇ È¥ÀÔÀÌ Àû¾î, in vivo ½ÇÇèÀ» À§ÇÑ AAV Á¤Á¦½ÇÇèÀ̳ª cell infection µî ÈÄ¼Ó ½ÇÇè¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë

1.

AAV Extraction Solution A

25 §¢

2.

AAV Extraction Solution B

2.5 §¢

¹è¾ç¿ë±âº° ½Ã¾à »ç¿ë¿ë·®

 

¹èÁö
volume

0.5 M EDTA
(pH8.0)

AAV Extraction
Solution A

AAV Extraction
Solution B

6 cm dish

4 §¢

50 §¡

200 §¡

20 §¡

10 cm dish

10 §¢

125 §¡

500 §¡

50 §¡

15 cm dish

26 §¢

325 §¡

1,300 §¡

130 §¡

T25 flask

4 §¢

50 §¡

250 §¡

25 §¡

T75 flask

13 §¢

162.5 §¡

650 §¡

65 §¡

T225 flask

40 §¢

500 §¡

2,000 §¡

200 §¡

º¸Á¸ ½Ç¿Â
Àû¿ë ¿¹ - Freeze/thaw¹æ¹ý°ú ÃßÃâÈ¿À² ºñ±³
º» Á¦Ç°À» ÀÌ¿ëÇÏ¿© HEK293 cell (producing cell)·ÎºÎÅÍ ZsGreen 1À» Æ÷ÇÔÇÑ AAV2¸¦ ÃßÃâÇÏ¿´´Ù. AAV2 Á¦ÀÛ¿¡´Â AAVpro Helper Free System(AAV2) (Code 6230)¹× pAAV-ZsGreen1 Vector (Code 6231)¸¦ »ç¿ëÇÏ¿´´Ù.
Freeze/thaw¹æ¹ýÀ» ºñ±³±ºÀ¸·Î °¢°¢ Real Time PCR¿¡ ÀÇÇÑ vector genome Á¤·® ¹× HT1020 cell infectionÀ» ÅëÇÑ biological titer¸¦ ÃøÁ¤ÇÑ °á°ú, AAVpro¢ç Extraction SolutionÀ» ÀÌ¿ëÇÏ¿© ÃßÃâÇÑ AAV2°¡ ´õ ³ôÀº titer¸¦ ³ªÅ¸³»´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù.


±×¸²1. AAV2 extraction ÃßÃâÈ¿À² ºñ±³ <Vector Genome Á¤·®>


±×¸²2. AAV2 extraction ÃßÃâÈ¿À² ºñ±³ <biological titer ÃøÁ¤>
°ü·ÃÁ¦Ç°

Keyword :

Adeno-Associated Virus (AAV)
>
Adeno-associated virus Á¦ÀÛ¿¡ ÃÖÀûÀÎ packagi..
Packaging (293T Cell Line)
>
Helper Free ½Ã½ºÅÛ¿¡ ÀÇÇÑ ´Ù¾çÇÑ serotypeÀÇ ..
AAV1, 2, 5, 6 Vector System
>
Cre-Recombinase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, ..
CRE Recombinase System
>
¥â-galactosidase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2,..
LacZ System
>
Çü±¤´Ü¹éÁúÀÌ Æ÷ÇÔµÈ AAV vector
pAAV-ZsGreen1 Vector
>
Adeno-associated virus delivery-CRISRP/Cas9 ..
CRISPR/Cas9 Vector System
>
SaCas9¸¦ ÀÌ¿ëÇÑ AAV mediated CRISPR Genome E..
CRISPR/SaCas9 Vector System (AAV2)
>
AAV2 Delivery Tet À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Inducible System (AAV2)
>
Cell°ú ¹è¾ç ¹èÁö¿¡¼­ AAV ÃßÃâºÎÅÍ Á¤Á¦±îÁö
Cell & Sup. Purification Kit
>
´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦¿¡ ÃÖÀû
Purification Kit Maxi/Midi (All Serotypes)
>
AAV Serotype 2 Á¤Á¦ kit
Purification Kit (AAV2 Àü¿ë)
>
AAV¸¦ È¿À²ÀûÀ¸·Î ³óÃà
AAVpro¢ç Concentrator
>
ºü¸£°í Á¤È®ÇÑ AAV ¿ª°¡ÃøÁ¤ (qPCR)
AAVpro¢ç Titration Kit (for Real Time PCR)
>
°£ÆíÇÑ AAV ÃßÃâ½Ã¾à
Extraction Solution
>
±âÁ¸ÀÇ AAV ÃßÃâ ¹æ¹ýÀ¸·Î Á¤Á¦±îÁö 4½Ã°£ ³» ..
AAV Freeze-thaw extraction solution