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조혈모세포, 줄기세포 등의 Lentiviral transduction을 위한 EF1α promoter

EF1 α promoter 벡터 시스템

제조사	제품코드	제품명	용량	가격 (부가세별도)	사용자매뉴얼
Clontech	631253	Lenti-X™ Expression System (EF1a Version)	set (Package)	2,225,000원	, ,
Clontech	631983	pLVX-EF1a-AcGFP1-N1 Vector	10 µg	1,352,000원	,
Clontech	631984	pLVX-EF1a-AcGFP1-C1 Vector	10 µg	1,352,000원	,
Clontech	631985	pLVX-EF1a-mCherry-C1 Vector	10 µg	1,352,000원	,
Clontech	631986	pLVX-EF1a-mCherry-N1 Vector	10 µg	1,352,000원	,
Clontech	631989	pLVX-EF1a-DsRed-Monomer-C1	10 µg	1,352,000원	,
Clontech	631990	pLVX-EF1a-DsRed-Monomer-N1 Vector	10 µg	1,352,000원	,
Clontech	631982	pLVX-EF1a-IRES-ZsGreen1 Vector	10 µg	1,291,000원	,
Clontech	631987	pLVX-EF1a-IRES-mCherry Vector	10 µg	1,352,000원	,

Product index

If CMV promoters are silenced in your cell type, try EF-1 alpha
High-titer lentiviral vector system
Constitutive expression
Robust, long-term expression, in the broadest range of cell types

Advantages of the EF-1 Alpha Promoter

Lenti-X™ lentiviral vector systems with EF-1 alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. The EF-1 alpha promoter, which offers a broad host range, is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic elongation factor 1 (1, 2).

Expression Options of Lentiviral Vectors with an EF-1 Alpha Promoter

The Lenti-X Expression System (EF1a Version) is a complete system that includes the pLVX-EF1a-IRES-Puro Vector (Figure 1), and our 4th Generation Lentiviral Packaging System. Alternatively, you can express your protein fused to a red (mCherry or DsRed-Monomer) or green (AcGFP1) fluorescent protein, or express your protein and a fluorescent protein separately from an internal ribosome entry site (IRES).

Figure 1. Lentiviral Vector Systems with an EF-1 Alpha Promoter.

References

1. Uetsuki, T., Naito, A., Nagata, S., and Kaziro, Y. (1989). Isolation and Characterization of the Human Chromosomal Gene for Polypeptide Chain Elongation Factor-1. J. Biol. Chem. 264:5791?5798.
2. Kim, D., Uetsuki, T., Kaziro, Y., Yamaguchi, N. and Sugano, S. (1990).Use of the human elongation factor 1α promoter as a versatile and efficient expression system. Gene 91:217?223.

Components

Lenti-X™ Expression System (EF1a Version) (Cat. No. 631253)

pLVX-EF1α-IRES-Puro Vector
Lenti-X™ Packaging single shots
Lenti-X™ GoStix Plus

Keyword : lenti,lentivirus,virus,ef1,ef-1,ef,렌티,바이러스

Lentivirus

[개요] Packaging Single Shots

[FAQ] Lentiviral Gene Delivery

Lentiviral expression vector를 포함한 시스템..

CMV promoter 벡터 시스템

조혈모세포, 줄기세포 등의 Lentiviral transdu..

EF1 α promoter 벡터 시스템

SARS-CoV-2-pseudotyped lentivirus 생산

Lenti-X™ SARS-CoV-2 Packaging Mix

임상 치료제 개발을 위한

3세대 Lentivirus packaging system

다양한 형광단백질 리포터를 포함한 Lentiviral..

형광단백질 리포터 벡터

Tetracycline 존재 하에서만 목적 단백질을 발..

Tet-on inducible Expression (Two vectors)

Tetracycline 존재 하에서만 목적 단백질을 발..

Tet One Inducible Expression (One vector)

Lentivirus를 이용한 sgRNA, Cas9 gene deliver..

Lenti-X™ CRISPR/Cas9 System

Tetracycline 존재 하에서만 목적 단백질을 발..

(Legacy) Tet-Inducible expression 시스템

Pormoterless vector로 pomorter 활성 연구

Reporter assay 시스템 (Promoterless vector)

Transfection 시약을 포함하여 더 간단한 packa..

Packaging single shots

239T, HEK-293 Cell보다 더 높은 packaging 효..

Packaging Cell Lines

1 step qRT-PCR로 역가를 빠르고 정확하게 측정

Lenti-X™ qRT-PCR Titration Kit

p24 ELISA로 빠르고 정확하게 역가 측정

Lenti-X™ p24 Rapid Titer Kit (Single Wash)

스마트폰으로 10분만에 Lentivirus 역가(Titer)..

GoStix™ Plus

Lentivirus 농도가 낮을 때, Mix & Spin으로 간..

Lentivirus 농축 시약

In vivo Lentiviral delivery 적용 시, 필수적..

Lentivirus 정제

Ecotropic lentivirus를 human cell에 적용

Ecotropic Receptor Booster

Lentivirus나 MMLV retrovirus의 더 빠른 trans..

Transduction 시약

목적 세포의 유전자에 삽입된 Provirus copy nu..

Provirus Quantitation Kit

Lentivirus가 목적세포 유전자에 삽입된 위치를..

Integration Site Analysis Kit

최적화된 Transfection Reagent & Packaging Mi..

TransIT® Lentivirus System

EF1 α promoter 벡터 시스템

Advantages of the EF-1 Alpha Promoter

Expression Options of Lentiviral Vectors with an EF-1 Alpha Promoter

References

Components

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