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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Lentivirus > EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transductionÀ» À§ÇÑ EF1¥á promoter

EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631253
Lenti-X¢â Expression System (EF1a Version)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
set
(Package)
2,225,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32774, x33433, x33847
Clontech
631983
pLVX-EF1a-AcGFP1-N1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 µg
1,352,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33429, x33843
Clontech
631984
pLVX-EF1a-AcGFP1-C1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 µg
1,352,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33428, x33842
Clontech
631985
pLVX-EF1a-mCherry-C1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 µg
1,352,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33435, x33849
Clontech
631986
pLVX-EF1a-mCherry-N1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 µg
1,352,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33436, x33850
Clontech
631989
pLVX-EF1a-DsRed-Monomer-C1
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 µg
1,352,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33430, x33844
Clontech
631990
pLVX-EF1a-DsRed-Monomer-N1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 µg
1,352,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33431, x33845
Clontech
631982
pLVX-EF1a-IRES-ZsGreen1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 µg
1,291,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33434, x33848
Clontech
631987
pLVX-EF1a-IRES-mCherry Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
10 µg
1,352,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33432, x33846

  • If CMV promoters are silenced in your cell type, try EF-1 alpha
  • High-titer lentiviral vector system
  • Constitutive expression
  • Robust, long-term expression, in the broadest range of cell types
Advantages of the EF-1 Alpha Promoter
Lenti-X¢â lentiviral vector systems with EF-1 alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. The EF-1 alpha promoter, which offers a broad host range, is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic elongation factor 1 (1, 2).
Expression Options of Lentiviral Vectors with an EF-1 Alpha Promoter
The Lenti-X Expression System (EF1a Version) is a complete system that includes the pLVX-EF1a-IRES-Puro Vector (Figure 1), and our 4th Generation Lentiviral Packaging System. Alternatively, you can express your protein fused to a red (mCherry or DsRed-Monomer) or green (AcGFP1) fluorescent protein, or express your protein and a fluorescent protein separately from an internal ribosome entry site (IRES).


Figure 1. Lentiviral Vector Systems with an EF-1 Alpha Promoter.
References
1. Uetsuki, T., Naito, A., Nagata, S., and Kaziro, Y. (1989). Isolation and Characterization of the Human Chromosomal Gene for Polypeptide Chain Elongation Factor-1. J. Biol. Chem. 264:5791?5798.
2. Kim, D., Uetsuki, T., Kaziro, Y., Yamaguchi, N. and Sugano, S. (1990).Use of the human elongation factor 1¥á promoter as a versatile and efficient expression system. Gene 91:217?223.
Components
Lenti-X¢â Expression System (EF1a Version) (Cat. No. 631253)
  • pLVX-EF1¥á-IRES-Puro Vector
  • Lenti-X¢â Packaging single shots
  • Lenti-X¢â GoStix Plus
°ü·Ã ÀÚ·á
LS&B47
: http://cms.takara.co.kr/file/lsnb/47-34-35.pdf




Keyword : lenti,lentivirus,virus,ef1,ef-1,ef,·»Æ¼,¹ÙÀÌ·¯½º

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