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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Lentivirus > TransIT¢ç Lentivirus System
ÃÖÀûÈ­µÈ Transfection Reagent & Packaging Mix

TransIT¢ç Lentivirus System

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 6650
TransIT¢ç Lentivirus System
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 rxns
°¡°Ý¹®ÀÇ   transit-lentivirus-system-full-protocol.pdf
Mirus
MIR 6655
TransIT¢ç Lentivirus System
°ü·ÃÇмú ±¸¸ÅÇϱâ
34 rxns
°¡°Ý¹®ÀÇ  
Mirus
MIR 6630
Lentivirus Packaging Mix Powered by MISSION¢ç
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 rxns
°¡°Ý¹®ÀÇ   transit-lentivirus-system-full-protocol.pdf
Mirus
MIR 6640
Lentivirus Packaging Mix Powered by MISSION¢ç
°ü·ÃÇмú ±¸¸ÅÇϱâ
34 rxns
°¡°Ý¹®ÀÇ  

  • Ÿ»ç ´ëºñ 2~3¹è ³ôÀº Functional titerÀÇ Lentivirus »ý»ê °¡´É
  • Ãß°¡ ³óÃàÀÌ ÇÊ¿ä¾ø´Â ³ôÀº ¿ª°¡ ½ÇÇö
  • ¹èÁö ±³È¯¾øÀÌ Virus ȸ¼ö±îÁö Çѹø¿¡ ÁøÇà
Á¦Ç°¼³¸í
MirusÀÇ TransIT¢ç Lentivirus SystemÀº MirusÀÇ Lentivirus »ý»êÀü¿ë Trans IT¢ç-Lenti Transfection Reagent ¿Í Lentivirus Packaging Mix Powered by MISSION¢ç Genomics°¡ Á¶ÇÕµÈ ÃÖ»óÀÇ Lentivirus packaging systemÀ¸·Î, ÃÖÀûÈ­µÈ ÇÁ·ÎÅäÄÝ°ú ÇÔ²² »ç¿ëÇÏ¿© ÃÖ´ë 108ÀÇ Lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù.
Á¦Ç°±¸¼º
TransIT¢ç Lentivirus System

Code

±¸¼ºÇ° ¸í

¿ë·®

º°µµ±¸¸Å½Ã
ÄÚµå

MIR 6650

TransIT¢ç-Lenti Transfection Reagent

0.3 §¢

MIR 6603

Lentivirus Packaging Mix Powered by MISSION¢ç

5 rxn (0.25 §¢)

MIR 6630

TransduceIT¢â Reagent

1 §¢

MIR 6620

MIR 6655

TransIT¢ç-Lenti Transfection Reagent

1.5 §¢

MIR 6600

Lentivirus Packaging Mix Powered by MISSION¢ç

34 rxn (1.7 §¢)

MIR 6640

TransduceIT¢â Reagent

1 §¢

MIR 6620

º¸Á¸ : - 20¡É
½ÇÇè ¿¹ : Ÿ»ç ´ëºñ ¸Å¿ì ³ôÀº functional titer·Î Lentivirus »ý»ê °¡´É


Up to 100-fold Increase in Functional Lentivirus Titers. Adherent 293T/17 cells were transfected in a 6-well plate with the pLKO.1-puro-CMV-TurboGFP¢â transfer vector and the Lentivirus Packaging Mix Powered by MISSION¢ç Genomics or pLenti6.2-GW/EmGFP transfer vector and the ViraPower¢â Lentiviral Packaging Mix (1:1 ration, 2 §¶/well) with the following reagents: TransIT¢ç-Lenti (3:1, vol:wt), Lipofectamine¢ç 2000 (3:1), or Lipofectamine¢ç 3000 (3:1:1). The supernatant was harvested, filtered (0.45 ¥ìm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 §¶/§¢ TransduceIT¢â and GFP expression was measured 72 hours post-transduction using guava easyCyte¢â 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.
°ü·ÃÁ¦Ç°
- Retrovirus/Lentivirus Transduction enhancer: RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
- °íÈ¿À² Lentivirus packaging cell line: Lenti-X¢â 293T Cell Line
- Mycoplasma ¿À¿°È®ÀÎ: MycoAlert¢â PLUS Mycoplasma detection kit




Keyword :

Lentivirus
>
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
>
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
>
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
>
ÀÓ»ó Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ
3¼¼´ë Lentivirus packaging system
>
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
>
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
>
Tetracycline Á¸Àç ÇÏ¿¡¼­¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
>
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
>
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
>
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
>
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
>
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
>
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
>
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
>
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
>
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
>
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
>
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
>
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
>
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
>
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
>
ÃÖÀûÈ­µÈ Transfection Reagent & Packaging Mi..
TransIT¢ç Lentivirus System