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AAV Serotype 2 Á¤Á¦ kit

Purification Kit (AAV2 Àü¿ë)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
6232
AAVpro¢ç Purification Kit (AAV2)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
2 ȸ
595,200¿ø 
744,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6232_e.v1509Da.pdf

  • AAV2 Àü¿ë Á¤Á¦ kit
  • µ¶ÀÚÀûÀÎ AAV ÃßÃâ¹ýÀ¸·Î Freeze/thaw, sonication ºÒÇÊ¿ä (ƯÇã Ãâ¿ø Áß)
  • Producing cell ¿¡¼­ÀÇ AAV ÃßÃâºÎÅÍ Á¤Á¦¿¡ ÇÊ¿äÇÑ ¸ðµç component Æ÷ÇÔ
Á¦Ç° ¼³¸í
Adeno-Associated Virus (AAV)´Â ºÐ¿­, ºñºÐ¿­ ¼¼Æ÷¿¡ À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇϸç, ƯÈ÷ ºñºÐ¿­ ¼¼Æ÷¿¡ ´ëÇØ Àå±â°£ À¯ÀüÀÚ ¹ßÇöÀÌ °¡´ÉÇϸç ƯÈ÷, ¸é¿ª¿ø¼º (immune response)ÀÌ ³·¾Æ in-vivo ½ÇÇè¿¡ ÃÖÀûÈ­µÈ virusÀÌ´Ù.
in-vivo ½ÇÇè¿¡ Àû¿ëÇÏ´Â °æ¿ì, µ¶¼ºÀ» °¨¼Ò½ÃÅ°±â À§ÇÏ¿© Packaging cell line ¶Ç´Â ¹èÁö À¯·¡ÀÇ ºÒ¼ø¹°À» Á¦°ÅÇÏ¿© °í¼øµµ·Î Á¤Á¦µÈ AAV¸¦ »ç¿ëÇÏ´Â °ÍÀº ¸Å¿ì Áß¿äÇÏ´Ù. ¶ÇÇÑ, culture cellÀÇ °æ¿ì¿¡µµ AAV¸¦ Á¤Á¦ÇÏ¿© »ç¿ëÇÏ¸é ºÒ¼ø¹°¿¡ ÀÇÇÑ ¿µÇâÀ» ÃÖ¼ÒÈ­ÇÒ ¼ö ÀÖ´Ù.

º» Á¦Ç°Àº AAV serotype 2¸¦ °£ÆíÇÏ°Ô Á¤Á¦ÇÒ ¼ö ÀÖ´Â kit·Î ultracentrifugation µîÀÇ º¹ÀâÇÑ °úÁ¤¾øÀÌ ¾à 4½Ã°£À̸é AAV2¸¦ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°¿¡¼­´Â µ¶ÀÚÀûÀÎ AAV extraction ¹æ¹ýÀ» »ç¿ëÇϹǷΠFreeze/thaw¹ý, sonication¹ý°ú °°ÀÌ ¹ø°Å·Î¿î Á¶ÀÛÀÌ ºÒÇÊ¿äÇÏ°í, ÀÚü °³¹ßÇÑ AAV Á¤Á¦¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© producing cell À¯·¡ ´Ü¹éÁúÀ̳ª ÇÙ»êÀÇ È¥ÀÔÀ» ¾ïÁ¦ÇÏ°í È¿À²ÀûÀ¸·Î AAV2¸¦ ÃßÃâÇÒ ¼ö ÀÖ´Ù. µû¶ó¼­ Nuclease ó¸® ¾øÀ̵µ dsDNAÀÇ È¥ÀÔÀ» Å©°Ô ÁÙÀÏ ¼ö ÀÖ´Ù.
Workflow
Fast and simple AAV2 purification - Á¦Ç°»ç¿ë µ¿¿µ»ó
³»¿ë

1.

AAV Extraction Solution A

14 §¢¡¿ 2°³

2.

AAV Extraction Solution B

2.8 §¢

3.

SD Solution*1

2.8 §¢

4.

Equilibration Buffer

11 §¢¡¿ 2°³

5.

Washing Buffer

11 §¢¡¿ 2°³

6.

Elution Buffer

7 §¢

7.

Suspension Buffer

1.2 §¢

8.

Prepacked Column*2

2°³

9.

Filter Device

2°³

10.

Collection Tube

4°³


*1 Àú¿Â º¸Á¸¿¡ ÀÇÇØ ¹é»ö ħÀü¹°ÀÌ »ý±â´Â °æ¿ì, 37¡É¿¡¼­ ¿ÏÀüÈ÷ ¿ëÇØ ÈÄ »ç¿ëÇϼ¼¿ä.
*2 °³ ´ç T225 flask 5Àå ºÐ·®ÀÇ Á¤Á¦¸¦ ±ÇÀåÇÕ´Ï´Ù.
¡Ø Nuclease¸¦ ó¸®ÇÏ´Â °æ¿ì, ¾Æ·¡ÀÇ ½Ã¾àÀ» ±ÇÀåÇÕ´Ï´Ù.
- 1 M MgCl2¿ë¾×
- Cryonase Cold-active Nuclease (Code 2670A/B)
º¸Á¸ 4¡É
°ü·ÃÁ¦Ç°

Keyword :

Adeno-Associated Virus (AAV)
>
Adeno-associated virus Á¦ÀÛ¿¡ ÃÖÀûÀÎ packagi..
Packaging (293T Cell Line)
>
Helper Free ½Ã½ºÅÛ¿¡ ÀÇÇÑ ´Ù¾çÇÑ serotypeÀÇ ..
AAV1, 2, 5, 6 Vector System
>
Cre-Recombinase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, ..
CRE Recombinase System
>
¥â-galactosidase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2,..
LacZ System
>
Çü±¤´Ü¹éÁúÀÌ Æ÷ÇÔµÈ AAV vector
pAAV-ZsGreen1 Vector
>
Adeno-associated virus delivery-CRISRP/Cas9 ..
CRISPR/Cas9 Vector System
>
SaCas9¸¦ ÀÌ¿ëÇÑ AAV mediated CRISPR Genome E..
CRISPR/SaCas9 Vector System (AAV2)
>
AAV2 Delivery Tet À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Inducible System (AAV2)
>
Cell°ú ¹è¾ç ¹èÁö¿¡¼­ AAV ÃßÃâºÎÅÍ Á¤Á¦±îÁö
Cell & Sup. Purification Kit
>
´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦¿¡ ÃÖÀû
Purification Kit Maxi/Midi (All Serotypes)
>
AAV Serotype 2 Á¤Á¦ kit
Purification Kit (AAV2 Àü¿ë)
>
AAV¸¦ È¿À²ÀûÀ¸·Î ³óÃà
AAVpro¢ç Concentrator
>
ºü¸£°í Á¤È®ÇÑ AAV ¿ª°¡ÃøÁ¤ (qPCR)
AAVpro¢ç Titration Kit (for Real Time PCR)
>
°£ÆíÇÑ AAV ÃßÃâ½Ã¾à
Extraction Solution
>
±âÁ¸ÀÇ AAV ÃßÃâ ¹æ¹ýÀ¸·Î Á¤Á¦±îÁö 4½Ã°£ ³» ..
AAV Freeze-thaw extraction solution