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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Retrovirus > Receptor Boosters (Ecotropic and Amphotropic)

Receptor Boosters (Ecotropic and Amphotropic)

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Clontech
631471
Ecotropic Receptor Booster
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20 ȸ
615,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33178

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  • Ecotropic/Amphotropic lentivirus³ª retrovirusÀ» ÀÌ¿ëÇÏ¿© Ÿ°Ù ¼¼Æ÷(Human cellÀ» Æ÷ÇÔ)¿¡ transduce½Ãų ¶§ »ç¿ë
  • ³ôÀº ÇüÁúµµÀÔ È¿À²- ÀϽÃÀûÀ¸·Î ¸ñÀû cellÀÇ viral receptor ÀÇ ³óµµ¸¦ Áõ°¡ ½ÃÄÑ´Â ¿ø¸®
  • °£´ÜÇÑ ÇÁ·ÎÅäÄÝ- virus¸¦ intectioin½ÃÅ°±â 2½Ã°£ ÀÌÀü¿¡ Ÿ°Ù ¼¼Æ÷¿Í receptor booster(concentrated exosome-like vesicles)¸¦ pre-incubation
Applications

±×¸². Receptor Boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with virus. Boosters result in increased transduction efficiency because treatment increases the cell surface density of the receptor recognized by the lentivirus (ecotropic) or retrovirus (ecotropic or amphotropic). Using this technology, for example, you can coat human cells with the ecotropic receptor (which is otherwise absent), enabling them to be transduced with ecotropic retrovirus or ecotropic lentivirus.


±×¸². Usually, it is not possible to infect Jurkat cells, human mesenchymal stem cells, or human primary neural progenitor cells with ecotropic pseudotyped lentivirus. However, pre-treatment of these cell types with Ecotropic Receptor Booster allows for very efficient transduction.


±×¸². Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used. Panel A. HT1080 cells were plated in 6-well plates 24 hr prior to transduction and incubated with 10 ¥ìl Ecotropic Receptor Booster for 2 hr. Cells were then transduced with Lenti-X ZsGreen1 lentivirus (MOI=~15) produced using Clontech's Lenti-X HTX Ecotropic Packaging System and assayed 48 hr later for ZsGreen1 expression. Panel B. A variety of human cell types that are normally resistant to transduction by ecotropic-pseudotyped lentiviral vectors were rendered susceptible to transduction by pre-treatment with the Ecotropic Receptor Booster
Recommended for Producing Ecotropic Pseudotyped Virus:

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