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Gene transfer (virus)
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Adenovirus
> Hexon ±â¹Ý titer ÃøÁ¤
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Hexon ±â¹Ý titer ÃøÁ¤
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Clontech
632250
Adeno-X Rapid Titer Kit
120 ȸ
(Package)
901,600¿ø
1,127,000¿ø
11.01 ~ 12.27
,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
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Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
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Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
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RetroNectin¢ç (Recombi..
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Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
RetroNectin¢ç Pro (Rec..
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Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
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Retrovirus vector £¦ ..
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Retro-X Q vector
pDON-5 DNA
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pMEI-5 DNA
Retrovirus Packaging K..
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Titer Set (for Real Ti..
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Adenovirus
Adeno-X¢â workflow
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Adenovirus Á¤Á¦ (Maxi ..
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qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
4 ½Ã°£ À̳»·Î ½Å¼ÓÇÏ°Ô ¹ÙÀÌ·¯½º ¿ª°¡ ÃøÁ¤
Plaque assay ¶Ç´Â End-point dilution assayº¸´Ù ´õ ºü¸£°í Á¤È®
Adeno-X Rapid Titer Kit
Adeno-X Rapid Titer Kit´Â Ç×ü¸¦ ÀÌ¿ëÇÏ¿© 1 ¼¼´ë E1-deleted adenovirus¿ª°¡¸¦ °£´ÜÇÏ°í ½Å¼ÓÇÏ°Ô ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù (2). º» Á¦Ç°Àº °¨¿°µÈ ¼¼Æ÷¸¦ hexon ƯÀÌÀû Ç×ü·Î ¿°»öÇÏ´Â ¹æ¹ýÀ» ±â¹ÝÀ¸·Î ÇÏ°í ÀÖ´Ù. Hexon ´Ü¹éÁúÀº adenoviral genomeÀ¸·Î À¯µµµÇ¸ç adenovirus º¹Á¦¿¡ ÇÊ¿äÇÑ adenoviral capsidÀÇ Çʼö ±¸¼º ¿ä¼ÒÀÌ´Ù. ±×·¯³ª hexon ´Ü¹éÁúÀÇ ¹ßÇöÀº E1 À¯ÀüÀÚ »ê¹°¿¡ ÀÇÁ¸ÀûÀÌ´Ù. µû¶ó¼ °¨¿°µÈ ¼¼Æ÷¸¸ÀÌ hexon ´Ü¹éÁúÀ» »ý»êÇϱ⠶§¹®¿¡ HEK293°ú °°Àº E1 trans-complementing ¼¼Æ÷ (3)´Â °¨¿°µÈ È°¼ºÀ» ÃøÁ¤Çϴµ¥ »ç¿ëµÉ ¼ö ÀÖ´Ù. Rapid Titer °ËÃâ¹ýÀº ½ÇÇè ÁغñÀ» À§ÇÏ¿© 1~2 ½Ã°£, ±×¸®°í 2 ÀÏ ÈÄ °¨¿°µÈ ¼¼Æ÷¸¦ Ç¥½Ä, ¿°»öÇÏ°í ÃøÁ¤Çϴµ¥ Ãß°¡ 3 ½Ã°£ Á¤µµ ¼Ò¿äµÈ´Ù. °¢ ¿°»öµÈ ¼¼Æ÷´Â single infection unit¿Í ÀÏÄ¡ÇÑ´Ù (Figure 2).
Figure 2. With the Adeno-X Rapid Titer Kit, infected cells turn brown, so they are easily counted using a microscope.
Healthy HEK 293 cells (5 x 10
5
/ml) were added to each well (1 ml/well) of a 12-well culture plate and then infected with various dilutions of an adenovirus stock according to the Adeno-X Rapid Titer Protocol. Following a 48 hr incubation, infected cells were identified by adding mouse anti-hexon antibody, HRP-conjugated rat anti-mouse antibody, and DAB substrate. This microscope image, taken with a 20X objective, shows a field of infected cells in a well that received a 10-5 viral dilution.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Obtain Adenoviral Titers in Less than 4 hours (2009) Clontechniques XXIV(2):12-13.
2. Bewig, B. & Schmidt, W. E. (2000) Biotechniques 28(5):870-873.
3. Graham, F. L. et al. (1977) J. Gen. Virol. 36(1):59-74.
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Adenovirus
Adeno-X¢â workflow
Adenoviral Expression System 3
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi type)
Adenovirus Á¤Á¦ (Mega type)
Real Time PCR·Î Genome copy ¼ö¸¦ ½Å¼ÓÇÏ°Ô Ãø..
qPCR titer ÃøÁ¤ Kit
¸é¿ª ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£¿¡ °¨¿° titer ÃøÁ¤
Hexon ±â¹Ý titer ÃøÁ¤
°£ÆíÇÑ ¿ª°¡ ÃæºÐ ¿©ºÎ °ËÃâ Å°Æ®
Adeno-X GoStix
CAR Receptor Booster